Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein

Single-stranded DNA aptamers interacting with fibrils of Saccharomyces cerevisiae Sup35p were obtained by the SELEX procedure. The specificity of interactions with Sup35p was investigated for 10 out of 40 selected aptamers. Nine aptamers were found to bind to the fibrillar but not to the monomeric f...

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Veröffentlicht in:	Molecular biology (New York) 2009-08, Vol.43 (4), p.626-631
Hauptverfasser:	Surina, E. R, Morozkina, E. V, Marchenko, A. N, Antipin, A. A, Mitkevich, O. V, Kushnirov, V. V, Ter-Avanesyan, M. D, Benevolensky, S. V
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers, Nucleotide - chemistry Biochemistry Biomedical and Life Sciences Deoxyribonucleic acid DNA DNA, Single-Stranded - chemistry Human Genetics Life Sciences Molecular biology Peptide Termination Factors - chemistry Polymers Prions - chemistry Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae Proteins - chemistry SELEX Aptamer Technique Structural-Functional Analysis of Biopolymers and Their Complexes Yeast
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Zusammenfassung:	Single-stranded DNA aptamers interacting with fibrils of Saccharomyces cerevisiae Sup35p were obtained by the SELEX procedure. The specificity of interactions with Sup35p was investigated for 10 out of 40 selected aptamers. Nine aptamers were found to bind to the fibrillar but not to the monomeric form of Sup35p. The dissociation constant of the aptamer-fibril complex ranged from 0.1 to 1.0 μM for different aptamers. The aptamers can be used to study the prion transformation of Sup35p.
ISSN:	0026-8933 0026-8984 1608-3245
DOI:	10.1134/S0026893309040153