Molecular genetics - step by step implementation in maize breeding

Objective of this report is to present partly results on the investigation of the possibilities to apply ionizing radiations (fast neutrons, gama-rays), and chemical mutagens to get maize and wheat mutants with increased amount and improved protein quality. Besides this approach in mutation breeding, results on the very early investigation of biochemical background of opaque-2 mutation including use of coupled cell - free RNA and protein synthesis containing components from both wild and opaque-2 maize genotypes will be presented. Partial results on opaque-2 gene incorporation in different genetic background are reviewed. Part of report is dealing with different classes of molecular markers application in maize genome polymorphism investigation.