Hypermethylation of ITGBL1 is associated with poor prognosis in acute myeloid leukemia

The current study was aimed to investigate integrin beta‐like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real‐time methylation‐specific polymerase chain reaction (PCR; RQ‐MSP) and bisulfite sequencing PCR (BSP) were performed to detect th...

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Veröffentlicht in:	Journal of cellular physiology 2019-06, Vol.234 (6), p.9438-9446
Hauptverfasser:	Lian, Xin‐Yue, Ma, Ji‐Chun, Zhou, Jing‐Dong, Zhang, Ting‐Juan, Wu, De‐Hong, Deng, Zhao‐Qun, Zhang, Zhi‐Hui, Li, Xi‐Xi, He, Pin‐Fang, Yan, Yang, Lin, Jiang, Qian, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Acute myeloid leukemia Adult Aged Aged, 80 and over Azacitidine - pharmacology Biomarkers Bisulfite Bone marrow Case-Control Studies Demethylation DNA methylation DNA Methylation - drug effects DNA Methylation - genetics expression Female Follow-Up Studies Gene Expression Regulation, Leukemic - drug effects Humans Induction therapy Integrin beta1 - genetics Integrin beta1 - metabolism ITGBL1 K562 Cells Leukemia Leukemia, Myeloid, Acute - genetics Male Medical prognosis Methylation Middle Aged Multivariate Analysis Myeloid leukemia Patients Polymerase chain reaction Prognosis Promoter Regions, Genetic - genetics Promyeloid leukemia Remission Survival Survival Analysis Transcription Young Adult
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Zusammenfassung:	The current study was aimed to investigate integrin beta‐like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real‐time methylation‐specific polymerase chain reaction (PCR; RQ‐MSP) and bisulfite sequencing PCR (BSP) were performed to detect the methylation of ITGBL1 promoter. Real‐time quantitative PCR (RT‐qPCR) was performed to analyze ITGBL1 transcript level. The results showed that ITGBL1 methylation level in 131 patients with AML was significantly higher than 29 controls (p
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.27629