Relationship between genome packaging and Gag translation/AUG of primate lentiviruses

About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this...

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Veröffentlicht in:	Microbes and infection 2019-03, Vol.21 (2), p.119-123
Hauptverfasser:	Sakuragi, Sayuri, Shioda, Tatsuo, Sakuragi, Jun-ichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Codon, Initiator - genetics Gene Products, gag - genetics Genome dimerization HEK293 Cells HIV HIV-1 - genetics HIV-1 - physiology HIV-2 - genetics HIV-2 - physiology Humans Lentivirus Lentiviruses, Primate - genetics Lentiviruses, Primate - physiology Mutation Packaging Protein Biosynthesis Simian Immunodeficiency Virus - genetics Simian Immunodeficiency Virus - physiology SIV Translation Virus Assembly - genetics
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Beschreibung
Zusammenfassung:	About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation.
ISSN:	1286-4579 1769-714X
DOI:	10.1016/j.micinf.2018.09.002