In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

Pollen tubes require functional mitochondria in order to achieve fast and sustained growth. In addition, cell wall expansion requires a calcium gradient in the tube apex formed by a dedicated array of calcium pumps and channels. Most studies have traditionally focused on the molecular aspects of cal...

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Veröffentlicht in:Protoplasma 2019-03, Vol.256 (2), p.503-509
Hauptverfasser: Flores-Herrera, Cesar, Preciado-Linares, Gisela, Gonzalez-Vizueth, Israel, Corona de la Peña, Norma, Gutiérrez-Aguilar, Manuel
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Sprache:eng
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Zusammenfassung:Pollen tubes require functional mitochondria in order to achieve fast and sustained growth. In addition, cell wall expansion requires a calcium gradient in the tube apex formed by a dedicated array of calcium pumps and channels. Most studies have traditionally focused on the molecular aspects of calcium interactions and transport across the pollen tube plasmalemma. However, calcium transients across mitochondrial membranes from pollen tubes are beginning to be studied. Here, we report the presence of a ruthenium red-sensitive mitochondrial calcium uniporter-like activity in tobacco pollen tubes with functional oxidative phosphorylation. The present study provides a framework to measure in situ specifics of mitochondrial transport and respiration in pollen tubes from different plants. The relevance of a mitochondrial calcium uniporter for pollen tube growth is discussed.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-018-1316-z