Influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone on mast cells

Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N -3-oxododecanoyl homoserine lactone (3OC 12 -HSL), exerts effects on mammalian cells and modulates host immune response. Mast cells (MCs) are strategically located in the tissues that are constantly exposed to external stimulus. Therefore, it is very much possible that 3OC 12 -HSL may interact with MCs. Little is known, however, about specific effects of 3OC 12 -HSL on MCs. To address this, we investigated the influence of 3OC 12 -HSL on cell viability, apoptosis, intracellular calcium and cytokine release in MCs. We found that at high concentrations (100 μM), 3OC 12 -HSL inhibited proliferation and induced apoptosis in P815. The 3OC 12 -HSL treatment significantly increased intracellular calcium release in both P815 and HMC-1. We also observed that 3OC 12 -HSL-induced histamine release and degranulation in HMC-1 cells. Furthermore, 3OC 12 -HSL-induced IL-6 production at lower concentrations (6.25–12.5 μM) but steadily reduced IL-6 production at high concentration (50–100 μM). These data demonstrate that P. aeruginosa 3OC 12 -HSL affects MCs function.