The Effect of Marathon Running on DNA Damage and Oxidative Stress in Human Peripheral Blood Cell

Objective: to investigate the DNA damage and the level of anti-oxidative status on human peripheral blood cell in different-time after Marathon running, and discuss the mechanism of DNA damage induced by endurance exercise. Method: We select seven healthy men athletes, who participated in International Marathon Match in 2007. The subjects are required to have a test at the time of twenty-four hours pre-exercise, also 2 hours, 24 hours and 48 hours after exercise. We investigate the DNA damage on human peripheral blood cell by using the single cell gel electrophoresis (SCGE) and detected the level of SOD, GSH and MDA in serum. Results: The results have shown that the DNA damage on peripheral blood cell significantly increased at 2h and 24h after exercise and the 2h is significant higher than the 24h. It reduces to the level of pre-exercise after 48 hours. The activity of SOD and the content of GSH and MDA in serum increase significantly at 2h compared with the pre-exercise, and SOD activity and MDA content are