Antimicrobial susceptibility and molecular typing of toxigenic clinical isolates of Clostridium difficile causing infections in the south of Spain

Antimicrobial susceptibility to 6 antimicrobial agents, PCR-ribotyping and molecular genetics of fluoroquinolone resistance was assessed in 70 toxigenic clinical isolates of C. difficile recovered from patients attended in a hospital in southern Spain with suspected Clostridium difficile infection. Moxifloxacin was the least active drug, mainly driven by the aminoacid substitution Thr82Ile in GyrA, while PCR-ribotype 078 was the most prevalent lineage identified and grouped several of the fluoroquinolone resistant isolates. •Antimicrobial susceptibility and molecular typing of C. difficile are performed.•Vancomycin, linezolid and tigecycline were the most active agents.•Ribotype 078 was the most prevalent (20%), followed by ribotype 014 (17%).•A high prevalence of fluorquinolone-resistant ribotype 078 is observed.•Thr82 to Ile in GyrA was found in the majority of the resistant isolates.