Stabilization of Foxp3 by Targeting JAK2 Enhances Efficacy of CD8 Induced Regulatory T Cells in the Prevention of Graft-versus-Host Disease

CD8 induced regulatory T cells (iTregs) have been identified to suppress alloreactive immune responses and expressed regulatory T cell (Treg) ontological markers as similar as CD4 iTregs. However, adoptive transfer of CD8 iTreg-based therapy is hampered by the instability of Treg specific-transcript...

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Veröffentlicht in:The Journal of immunology (1950) 2018-11, Vol.201 (9), p.2812-2823
Hauptverfasser: Iamsawat, Supinya, Daenthanasanmak, Anusara, Voss, Jessica Heinrichs, Nguyen, Hung, Bastian, David, Liu, Chen, Yu, Xue-Zhong
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Sprache:eng
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Zusammenfassung:CD8 induced regulatory T cells (iTregs) have been identified to suppress alloreactive immune responses and expressed regulatory T cell (Treg) ontological markers as similar as CD4 iTregs. However, adoptive transfer of CD8 iTreg-based therapy is hampered by the instability of Treg specific-transcription factor, Foxp3. As CD8 iTregs were previously demonstrated to possess superior tumor-killing ability to CD4 iTregs, adoptive transfer of stabilized CD8 iTregs would be a potential therapy to prevent tumor relapse during graft-versus-leukemia disease (GVHD) treatment. In the current study, we generated alloantigen reactive CD8 iTregs from JAK2 T cells and adoptively transferred them to MHC-mismatched and haploidentical murine models of allogeneic bone marrow transplantation. JAK2 CD8 iTregs not only attenuated GVHD but also preserved graft-versus-leukemia effect. Mechanistic analysis revealed that JAK2 CD8 iTregs upregulated natural Treg marker (neuropilin-1), and augmented DNA demethylation of CNS2 region within Foxp3 gene. These properties licensed JAK2 CD8 iTregs to retain high Foxp3 expression resulting in less conversion to type 1 CTLs; as a result, JAK2 CD8 iTregs were able to maintain their suppressive and cytolytic function. Thus, our findings provide a strong rationale and means to stabilize CD8 iTregs by targeting JAK2, and the stabilized CD8 iTregs exhibit therapeutic potential for alleviating GVHD and preserving the graft-versus-leukemia effect.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1800793