Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis
Objectives Periodontitis is a chronic inflammatory disease characterized by tooth-supporting tissue destruction, which is elicited by the host’s immune response triggered against periodonto-pathogen bacteria. During periodontal tissue destruction, extracellular matrix components are metabolized and...
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Veröffentlicht in: | Clinical oral investigations 2019-04, Vol.23 (4), p.1887-1894 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Objectives
Periodontitis is a chronic inflammatory disease characterized by tooth-supporting tissue destruction, which is elicited by the host’s immune response triggered against periodonto-pathogen bacteria. During periodontal tissue destruction, extracellular matrix components are metabolized and fragmented. Some extracellular matrix component-derived fragments, such as low-molecular-weight hyaluronan (LMW-HA), have potent immunogenic potential, playing a role as damage-associated molecular patterns (DAMPs) during activation of immune cells. Dendritic cells (DCs) play a central role in the host’s immune response displayed during periodontitis; thus, this study aimed to analyze whether LMW-HA has an immunostimulatory activity on DCs when stimulated with periodonto-pathogen bacteria.
Materials and methods
LMW-HA-treated and non-treated DCs were stimulated with
Aggregatibacter actinomycetemcomitans
or
Porphyromonas gingivalis
and the mRNA expression for cytokines tumor necrosis factor-α (TNF-alpha), interleukin-1β (IL-1B), interleukin-6 (IL-6), and interleukin-23 (IL-23A) was quantified by RT-qPCR. In addition, transcription factors interferon regulatory factor 4 (IRF4), interferon regulatory factor 8 (IRF8), neurogenic locus notch homolog protein 2 (NOTCH2), and basic leucine zipper ATF-like transcription factor 3 (BATF3), involved in DC activation, were analyzed.
Results
Higher expression levels of TNF-alpha, IL-1B, IL-6, and IL-23A were detected in LMW-HA-treated DCs after bacterial infection, as compared with non-treated DCs. When LMW-HA-treated DCs were infected with
A. actinomycetemcomitans
, higher levels of IRF4, NOTCH2, and BATF3 were detected compared with non-treated cells; whereas against
P. gingivalis
infection, increased levels of IRF4 and NOTCH2 were detected.
Conclusion
LMW-HA plays an immunostimulatory role on the immune response triggered by DCs during infection with
A. actinomycetemcomitans
or
P. gingivalis
.
Clinical relevance
Detection of extracellular matrix component-derived fragments produced during periodontal tissue destruction, such as LMW-HA, could explain at least partly unsuccessful periodontal treatment and the chronicity of the disease. |
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ISSN: | 1432-6981 1436-3771 |
DOI: | 10.1007/s00784-018-2641-5 |