Effects of Cd, Cu, Zn and their combined action on microbial biomass and bacterial community structure

Heavy metal pollution can decrease the soil microbial biomass and significantly alter microbial community structure. In this study, a long-term field experiment (5 years) and short-term laboratory experiment (40 d) were employed to evaluate the effects of heavy metals (Cd, Cu, Zn), and their combinations at different concentrations, on the soil microbial biomass and the bacterial community. The ranges of heavy metal concentration in the long-term and short-term experiments were similar, with concentration ranges of Cd, Cu and Zn of about 0.3–1.5, 100–500, and 150–300 mg kg−1, respectively. Microbial biomass decreased with increasing soil heavy metal concentrations in both the long-term and short-term experiments. The interaction between soil physicochemical factors (pH, TN, TC) and heavy metals (Cd, Cu, Zn) played a major role in change in the bacterial community in long-term polluted soil. In the laboratory experiment, although each heavy metal had an adverse effect on the microbial biomass and community structure, Cu appeared to have a greater role in the changes compared to Cd and Zn. However, the synergistic effects of the heavy metals were greater than those of the single metals and the synergistic effect between Cu and Cd was greater than that of Cu and Zn. [Display omitted] •Long- and short-term effects of heavy metals (HM) on soil microbes differ.•Bacterial community determined by soil properties and HM in field experiment.•Effect of Cu on microbial biomass and bacterial community greater than Zn and Cd.•Synergism between Cu and Cd greater than Cu and Zn on soil microbes. In a soil containing a combination of Cd, Cu and Zn, Cu has the major influence on microbial biomass and the bacterial community.