Oral vaccine based on a surface immunogenic protein mixed with alum promotes a decrease in Streptococcus agalactiae vaginal colonization in a mouse model

•The SIP mixed with Alum used as oral vaccine decreased GBS vaginal colonization in a mouse model.•IgG with opsonophagocytic activities was detected.•Oral vaccine induced a Th1-immune response. The Surface Immunogenic Protein (SIP) of Group B Streptococcus (GBS) had been described as a good target f...

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Veröffentlicht in:Molecular immunology 2018-11, Vol.103, p.63-70
Hauptverfasser: Diaz-Dinamarca, D.A., Soto, D.A., Leyton, Y.Y., Altamirano-Lagos, M.J., Avendaño, M.J., Kalergis, A.M., Vasquez, A.E.
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container_start_page 63
container_title Molecular immunology
container_volume 103
creator Diaz-Dinamarca, D.A.
Soto, D.A.
Leyton, Y.Y.
Altamirano-Lagos, M.J.
Avendaño, M.J.
Kalergis, A.M.
Vasquez, A.E.
description •The SIP mixed with Alum used as oral vaccine decreased GBS vaginal colonization in a mouse model.•IgG with opsonophagocytic activities was detected.•Oral vaccine induced a Th1-immune response. The Surface Immunogenic Protein (SIP) of Group B Streptococcus (GBS) had been described as a good target for vaccine development. To date, SIP has been reported as a highly conserved protein, and in a mouse model it induces protection against lethal GBS challenge. Also, similar effects have been described by intranasal immunization with a SIP-based vaccine. In this study, we show the immune response induced by an oral SIP-based vaccine formulated on alum in a mouse model. Our vaccine can reduce vaginal GBS colonization and induce specific SIP-antibodies with opsonophagocytosis activities against GBS. Moreover, we observed the activation of T-cells producing IFN-γ, TNF-α, IL-10, IL-2, and increased expression of the transcription factor T-bet, suggesting a Th1-type humoral response. The oral SIP-based vaccine is a novel alternative in the development of a vaccine against GBS.
doi_str_mv 10.1016/j.molimm.2018.08.028
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The Surface Immunogenic Protein (SIP) of Group B Streptococcus (GBS) had been described as a good target for vaccine development. To date, SIP has been reported as a highly conserved protein, and in a mouse model it induces protection against lethal GBS challenge. Also, similar effects have been described by intranasal immunization with a SIP-based vaccine. In this study, we show the immune response induced by an oral SIP-based vaccine formulated on alum in a mouse model. Our vaccine can reduce vaginal GBS colonization and induce specific SIP-antibodies with opsonophagocytosis activities against GBS. Moreover, we observed the activation of T-cells producing IFN-γ, TNF-α, IL-10, IL-2, and increased expression of the transcription factor T-bet, suggesting a Th1-type humoral response. 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subjects Oral vaccine
Streptococcus agalactiae
Surface immunogenic protein
Vaginal colonization
title Oral vaccine based on a surface immunogenic protein mixed with alum promotes a decrease in Streptococcus agalactiae vaginal colonization in a mouse model
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