Oral vaccine based on a surface immunogenic protein mixed with alum promotes a decrease in Streptococcus agalactiae vaginal colonization in a mouse model
•The SIP mixed with Alum used as oral vaccine decreased GBS vaginal colonization in a mouse model.•IgG with opsonophagocytic activities was detected.•Oral vaccine induced a Th1-immune response. The Surface Immunogenic Protein (SIP) of Group B Streptococcus (GBS) had been described as a good target f...
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Veröffentlicht in: | Molecular immunology 2018-11, Vol.103, p.63-70 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | •The SIP mixed with Alum used as oral vaccine decreased GBS vaginal colonization in a mouse model.•IgG with opsonophagocytic activities was detected.•Oral vaccine induced a Th1-immune response.
The Surface Immunogenic Protein (SIP) of Group B Streptococcus (GBS) had been described as a good target for vaccine development. To date, SIP has been reported as a highly conserved protein, and in a mouse model it induces protection against lethal GBS challenge. Also, similar effects have been described by intranasal immunization with a SIP-based vaccine. In this study, we show the immune response induced by an oral SIP-based vaccine formulated on alum in a mouse model. Our vaccine can reduce vaginal GBS colonization and induce specific SIP-antibodies with opsonophagocytosis activities against GBS. Moreover, we observed the activation of T-cells producing IFN-γ, TNF-α, IL-10, IL-2, and increased expression of the transcription factor T-bet, suggesting a Th1-type humoral response. The oral SIP-based vaccine is a novel alternative in the development of a vaccine against GBS. |
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ISSN: | 0161-5890 1872-9142 |
DOI: | 10.1016/j.molimm.2018.08.028 |