EVALUATION OF THE COMPARATIVE TOXICITY OF VARIOUS MATERIALS FOR DENTAL PROSTHETICS ON CELL CULTURE MODELS

Difficulties in repairing the defects of the teeth are related with allergic-inflammatory, traumatic and dystrophic complications arising from the interaction of the foreign body with the mucous tissues of the oral cavity after the patient's prosthesis is established. The aim of our study was to establish the toxic pro-inflammatory activity of materials used for the manufacturing of bases of removable dentures - plastics based on polymethylmethacrylate prosthetic complexes Prothyl Hot, Ftorax and Perflex Flexi Nylon on the model of human leukemia transformed T cells (Jurkat cells) and MDCK cells. For the cells simulation Jurkat and MDCK cells was incubated with the components of prosthetic materials, Prothyl Hot, Ftorax and Perflex Flexi Nylon/ Prosthetic materials were added to the incubation medium at the doses used in practice (calculated at 106 cells); duration of incubation was 24 hours. A comparative assessment of the toxicity of prosthetic materials was determined by the MTT test (activity of mitochondrial dehydrogenases). Statistical analysis was carried out using the package (SPSS version 11.0). The statistical reliability of the difference between the indices was evaluated by the Student t test (the P