2-deoxyglucose inhibits angiogenesis of rheumatoid arthritis via activating AMPK pathway

To observe the effects of 2-deoxyglucose inhibiting synovial pannus of adjuvant arthritis rats and to explore its potential mechanism of inhibiting angiogenesis by investigating proliferation, migration and matrigel tube formation assay . The effect of 2-DG on synovial pannus was evaluated by histop...

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Veröffentlicht in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2018-07, Vol.38 (8), p.962-968
Hauptverfasser: Wang, Ying, Wei, Yingmei, Cheng, Xiu, Sun, Xiaojin, Ma, Linyan, Song, Yining, Zhou, Jing, Wei, Fang, Liu, Hao
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Sprache:chi
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Zusammenfassung:To observe the effects of 2-deoxyglucose inhibiting synovial pannus of adjuvant arthritis rats and to explore its potential mechanism of inhibiting angiogenesis by investigating proliferation, migration and matrigel tube formation assay . The effect of 2-DG on synovial pannus was evaluated by histopathology of HE staining; HUVEC proliferation was determined by CCK-8 method; migration of FLS were determined by transwell; matrigel tube formation assay was made for assessing tube number of HUVEC; p-AMPK and Bcl-2 were detected by Western blot assay; AMPK signaling pathway in HUVEC was inhibited by compound C, which is an inhibitor of AMPK activation. 2-DG (200 mg/kg) obviously decreased appearance of synovial pannus ( < 0.01); , 2-DG (0.5 mmol/L and/or 5 mmol/L) obviously inhibited proliferation, migration and tube number of HUVEC ( < 0.01 or < 0.001), and its effects on HUVEC were reversed by using AMPK antagonist (Compound C); Western blot showed that 2-DG (5 mmol/L) increased expression of p-AMPK and decrease
ISSN:1673-4254
DOI:10.3969/j.issn.1673-4254.2018.08.10