The stimulatory effect of subluteal progesterone environment on the superovulatory response of passive immunization against inhibin in goats

The objective of the current study was to investigate the effect of subluteal progesterone environment on the efficacy of the ovarian responses in goats passively immunized against endogenous inhibin. Twelve cycling female Shiba goats were synchronized using two doses of prostaglandin F2α 11 days apart. After detection of ovulation (D0, by ultrasonography), goats were randomly assigned into 2 groups: (1) Subluteal P4 group (SLP; n = 6), treated with a previously once-used controlled intravaginal progesterone-releasing device (ou-CIDR) intravaginal from D 6 to D 12 concurrently with a luteolytic dose of PGF2α at D6 (2) Normal Luteal P4 group (NLP; n = 6), had neither ou-CIDR nor PGF2α. In both groups, goats were treated with an intravenous injection of 10 ml of inhibin antiserum at D10. After that, another luteolytic dose of PGF2α was administrated to goats in both groups at D12, and withdrawal of ou-CIDR in the SLP group. Detection of estrous behavior was checked again 24 h following PGF2α administration and or ou-CIDR withdrawal at 8 h intervals with a teaser buck. The follicle populations ≥4 mm in each goat were monitored through transrectal ultrasonography daily from 1 day after inhibin antiserum administration (0 h) and continued until ovulation had been detected (disappearance of large dominant follicles by ultrasonography). In addition, a repeated ultrasonographic scanning was performed 7 days after ovulation, and the ovulation rate had been estimated by counting the number of observed corpora lutea by ultrasonography. Blood samples were collected for measurement of plasma circulating inhibin binding activity, FSH, LH, estradiol (E2), and progesterone (P4) in both groups. Results revealed significant increases in maximum diameter of the preovulatory follicles (5.92 ± 0.17 mm vs 5.31 ± 0.26 mm; P