Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand

1 Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand 2 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3 Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Daejeon 305-806, Republic of Korea Correspondence Somboon Tanasupawat Somboon.T{at}chula.ac.th A Gram-positive, rod-shaped, strictly aerobic, spore-forming, moderately halophilic bacterium, designated strain SSK2-2 T , was isolated from fermented shrimp paste (ka-pi) produced in Thailand. It contained MK-7 as the predominant menaquinone and meso -diaminopimelic acid in the cell-wall peptidoglycan. The isolate grew at 8–43 °C, pH 6–9 and in 0.5–24 % (w/v) NaCl (optimum, 6–14 % NaCl). The major cellular fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . Phosphatidylglycerol and diphosphatidylglycerol were the major polar lipid components. The DNA G+C content was 39.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SSK2-2 T was most closely related to Oceanobacillus picturae KCTC 3821 T with 98.7 % sequence similarity. Based on phenotypic and molecular features combined with DNA–DNA hybridization results ( 24.9 % with O. picturae KCTC 3821 T ), this strain represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus kapialis sp. nov. is proposed; the type strain is SSK2-2 T (=KCTC 13177 T =PCU 300 T =TISTR 1858 T ). Abbreviations: DAP, diaminopimelic acid The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SSK2-2 T is AB366005 . A table showing the cellular fatty acid compositions of strain SSK2-2 T and related taxa is available as supplementary material with the online version of this paper.