Cytokine release kinetics of concentrated growth factors in different scaffolds

Objectives Concentrated growth factors (CGF) exhibit superior potential for periodontal tissue regeneration; however, little is known about the release pattern of CGF over time. This study was designed to investigate the in vitro release pattern of growth factors and cytokines from CGF in deproteinized bovine bone mineral (DBBM) and intrafibrillarly-mineralized collagen (IMC). Materials and methods CGF, CGF mixed with DBBM (CGF-DBBM), and CGF mixed with IMC (CGF-IMC) were cultured in vitro for 28 days and media supernatants were collected after 24 h, 72 h, and 7, 14, 21, and 28 days respectively. The factors investigated included platelet-derived growth factor-BB, transforming growth factor beta 1, vascular endothelial growth factor, insulin-like growth factor 1, basic fibroblast growth factor, C3a, and C5a. Results We found that CGF-IMC released the highest total amount of cytokines compared to CGF and CGF-DBBM ( p