Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells

: To determine the effects of cysteinyl leukotriene receptors (CysLT R and CysLT R) on phagocytosis of mouse BV2 microglial cells. : BV2 cells were stimulated with microglial activators lipopolysaccharide (LPS) or CysLT receptor agonists LTD . The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry. The intracellular distributions of CysLT R and CysLT R in BV2 cells were examined with immunofluorescence staining. : Both LPS and LTD could significantly enhance the phagocytosis of BV2 cells, and such effect could be inhibited by CysLT R selective antagonist Montelukast and CysLT R selective antagonist HAMI 3379. The activation of BV2 cells induced by LTD or LPS resulted in changes in intracellular distributions of CysLT R and CysLT R. CysLT R and CysLT R was co-localization with a similar distribution. : CysLT R and CysLT R regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect.