Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther
Key message (1) We systematically analyze the promoter activities of AtCKLs in various tissues; (2) AtCKL2 and AtCKL7 were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of AtCKL2 and AtCKL7 expression under HT. High...
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| Veröffentlicht in: | Plant molecular biology 2018-09, Vol.98 (1-2), p.33-49 |
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| creator | Li, Yaoyao Min, Ling Zhang, Lin Hu, Qin Wu, Yuanlong Li, Jie Xie, Sai Ma, Yizan Zhang, Xianlong Zhu, Longfu |
| description | Key message
(1) We systematically analyze the promoter activities of
AtCKLs
in various tissues; (2)
AtCKL2
and
AtCKL7
were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of
AtCKL2
and
AtCKL7
expression under HT.
High temperature (HT) can seriously impede plant growth and development, causing severe loss of crop production. In
Arabidopsis, AtCKL
genes show high similarity to
GhCKI
, a gene reported to disrupt tapetal programmed cell death in cotton. However, most of
AtCKL
genes are not well characterized. Here, we systematically analyzed the expression patterns of
AtCKLs
in various tissues. The expression of
AtCKL2
and
AtCKL7
was induced in early anther development under HT, which is similar to the case of
GhCKI
. In silico analysis of
AtCKL2
and
AtCKL7
promoters indicated that four types of transcription factors (TFs) (MADS, NAC, WRKY and R2R3-MYB) might bind to
AtCKL2
and
AtCKL7
promoters. Furthermore, three MADS, three NAC, one WRKY, and three R2R3-MYB TFs were up-regulated in stage 1–8 anthers and three R2R3-MYB TFs were up-regulated in stage 9–10 anthers under HT, implying the important roles of R2R3-MYB genes in the response of anthers to HT. Among the R2R3-MYB genes,
AtMYB24
showed the similar expression as
AtCKL2
and
AtCKL7
in the anthers under HT. Additionally, yeast one-hybrid and dual-luciferase reporter system assays verified that AtMYB24 could bind to
AtCKL2
and
AtCKL7
promoters and activate the expression of these two genes. In brief, this study provides the overall expression profiles of
AtCKLs
, useful information for unraveling the molecular mechanism of
AtCKL2
and
AtCKL7
gene expression in early anther development under HT, and important clues for elucidating the mechanism of transcriptional regulation of
CKI
genes in plant anther under HT, which are critical to the reduction of crop yield loss resulting from HT. |
| doi_str_mv | 10.1007/s11103-018-0760-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2094420132</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2094420132</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-cc09484dc9a59517b7adac1e1cdc4f0fecad306faa00a03b1372cd75ce5a92bb3</originalsourceid><addsrcrecordid>eNp1kcFq3DAQhkVpaDZJH6CXIuilFzUzkm3tHsOSJoFAc0jOQpbHu9qsJVeyD337aNk0hUJOEuj7_hHzM_YF4QcC6MuMiKAE4FKAbkDoD2yBtVaiBrn8yBaAjRZVhfKUneW8AyiWaj6xUwVY1brRC-YfUhziRCnz2POrZFvfxTH7zNc2kw_82Ydy4Xdi75-JS25DxzV3MfSUeB7J-d47vvWbrZhoGCnZaU7EE-UxhiKWCBumLaULdtLbfabPr-c5e_p5_bi-Ffe_bu7WV_fCKS0n4RysqmXVuZWtVzXqVtvOOiR0nat66MnZTkHTWwtgQbVYLNfp2lFtV7Jt1Tn7fswdU_w9U57M4LOj_d4GinM2suRXElDJgn77D93FOYXyuwNVllQGYaHwSLkUc07UmzH5waY_BsEcejDHHkzpwRx6MLo4X1-T53ag7s34u_gCyCOQy1PYUPo3-v3UF9gqkxs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2093013061</pqid></control><display><type>article</type><title>Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Li, Yaoyao ; Min, Ling ; Zhang, Lin ; Hu, Qin ; Wu, Yuanlong ; Li, Jie ; Xie, Sai ; Ma, Yizan ; Zhang, Xianlong ; Zhu, Longfu</creator><creatorcontrib>Li, Yaoyao ; Min, Ling ; Zhang, Lin ; Hu, Qin ; Wu, Yuanlong ; Li, Jie ; Xie, Sai ; Ma, Yizan ; Zhang, Xianlong ; Zhu, Longfu</creatorcontrib><description>Key message
(1) We systematically analyze the promoter activities of
AtCKLs
in various tissues; (2)
AtCKL2
and
AtCKL7
were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of
AtCKL2
and
AtCKL7
expression under HT.
High temperature (HT) can seriously impede plant growth and development, causing severe loss of crop production. In
Arabidopsis, AtCKL
genes show high similarity to
GhCKI
, a gene reported to disrupt tapetal programmed cell death in cotton. However, most of
AtCKL
genes are not well characterized. Here, we systematically analyzed the expression patterns of
AtCKLs
in various tissues. The expression of
AtCKL2
and
AtCKL7
was induced in early anther development under HT, which is similar to the case of
GhCKI
. In silico analysis of
AtCKL2
and
AtCKL7
promoters indicated that four types of transcription factors (TFs) (MADS, NAC, WRKY and R2R3-MYB) might bind to
AtCKL2
and
AtCKL7
promoters. Furthermore, three MADS, three NAC, one WRKY, and three R2R3-MYB TFs were up-regulated in stage 1–8 anthers and three R2R3-MYB TFs were up-regulated in stage 9–10 anthers under HT, implying the important roles of R2R3-MYB genes in the response of anthers to HT. Among the R2R3-MYB genes,
AtMYB24
showed the similar expression as
AtCKL2
and
AtCKL7
in the anthers under HT. Additionally, yeast one-hybrid and dual-luciferase reporter system assays verified that AtMYB24 could bind to
AtCKL2
and
AtCKL7
promoters and activate the expression of these two genes. In brief, this study provides the overall expression profiles of
AtCKLs
, useful information for unraveling the molecular mechanism of
AtCKL2
and
AtCKL7
gene expression in early anther development under HT, and important clues for elucidating the mechanism of transcriptional regulation of
CKI
genes in plant anther under HT, which are critical to the reduction of crop yield loss resulting from HT.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/s11103-018-0760-7</identifier><identifier>PMID: 30145767</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Anthers ; Apoptosis ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Casein ; Casein kinase I ; Casein Kinase I - genetics ; Casein Kinase I - metabolism ; Cell death ; Cotton ; Crop production ; Crop yield ; Flowers - genetics ; Flowers - physiology ; Gene Duplication ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Genes, Plant ; Germination - genetics ; Glucuronidase - metabolism ; High temperature ; Hot Temperature ; Kinases ; Life Sciences ; Phylogeny ; Plant growth ; Plant Pathology ; Plant Sciences ; Promoter Regions, Genetic - genetics ; Promoters ; Reproduction - genetics ; Seedlings - genetics ; Seeds - genetics ; Temperature effects ; Transcription factors ; Transcription Factors - metabolism ; Yeasts</subject><ispartof>Plant molecular biology, 2018-09, Vol.98 (1-2), p.33-49</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Plant Molecular Biology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-cc09484dc9a59517b7adac1e1cdc4f0fecad306faa00a03b1372cd75ce5a92bb3</citedby><cites>FETCH-LOGICAL-c372t-cc09484dc9a59517b7adac1e1cdc4f0fecad306faa00a03b1372cd75ce5a92bb3</cites><orcidid>0000-0002-4947-8609</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11103-018-0760-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11103-018-0760-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30145767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yaoyao</creatorcontrib><creatorcontrib>Min, Ling</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Wu, Yuanlong</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Xie, Sai</creatorcontrib><creatorcontrib>Ma, Yizan</creatorcontrib><creatorcontrib>Zhang, Xianlong</creatorcontrib><creatorcontrib>Zhu, Longfu</creatorcontrib><title>Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><addtitle>Plant Mol Biol</addtitle><description>Key message
(1) We systematically analyze the promoter activities of
AtCKLs
in various tissues; (2)
AtCKL2
and
AtCKL7
were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of
AtCKL2
and
AtCKL7
expression under HT.
High temperature (HT) can seriously impede plant growth and development, causing severe loss of crop production. In
Arabidopsis, AtCKL
genes show high similarity to
GhCKI
, a gene reported to disrupt tapetal programmed cell death in cotton. However, most of
AtCKL
genes are not well characterized. Here, we systematically analyzed the expression patterns of
AtCKLs
in various tissues. The expression of
AtCKL2
and
AtCKL7
was induced in early anther development under HT, which is similar to the case of
GhCKI
. In silico analysis of
AtCKL2
and
AtCKL7
promoters indicated that four types of transcription factors (TFs) (MADS, NAC, WRKY and R2R3-MYB) might bind to
AtCKL2
and
AtCKL7
promoters. Furthermore, three MADS, three NAC, one WRKY, and three R2R3-MYB TFs were up-regulated in stage 1–8 anthers and three R2R3-MYB TFs were up-regulated in stage 9–10 anthers under HT, implying the important roles of R2R3-MYB genes in the response of anthers to HT. Among the R2R3-MYB genes,
AtMYB24
showed the similar expression as
AtCKL2
and
AtCKL7
in the anthers under HT. Additionally, yeast one-hybrid and dual-luciferase reporter system assays verified that AtMYB24 could bind to
AtCKL2
and
AtCKL7
promoters and activate the expression of these two genes. In brief, this study provides the overall expression profiles of
AtCKLs
, useful information for unraveling the molecular mechanism of
AtCKL2
and
AtCKL7
gene expression in early anther development under HT, and important clues for elucidating the mechanism of transcriptional regulation of
CKI
genes in plant anther under HT, which are critical to the reduction of crop yield loss resulting from HT.</description><subject>Anthers</subject><subject>Apoptosis</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Casein</subject><subject>Casein kinase I</subject><subject>Casein Kinase I - genetics</subject><subject>Casein Kinase I - metabolism</subject><subject>Cell death</subject><subject>Cotton</subject><subject>Crop production</subject><subject>Crop yield</subject><subject>Flowers - genetics</subject><subject>Flowers - physiology</subject><subject>Gene Duplication</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Genes, Plant</subject><subject>Germination - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>High temperature</subject><subject>Hot Temperature</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Phylogeny</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoters</subject><subject>Reproduction - genetics</subject><subject>Seedlings - genetics</subject><subject>Seeds - genetics</subject><subject>Temperature effects</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Yeasts</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kcFq3DAQhkVpaDZJH6CXIuilFzUzkm3tHsOSJoFAc0jOQpbHu9qsJVeyD337aNk0hUJOEuj7_hHzM_YF4QcC6MuMiKAE4FKAbkDoD2yBtVaiBrn8yBaAjRZVhfKUneW8AyiWaj6xUwVY1brRC-YfUhziRCnz2POrZFvfxTH7zNc2kw_82Ydy4Xdi75-JS25DxzV3MfSUeB7J-d47vvWbrZhoGCnZaU7EE-UxhiKWCBumLaULdtLbfabPr-c5e_p5_bi-Ffe_bu7WV_fCKS0n4RysqmXVuZWtVzXqVtvOOiR0nat66MnZTkHTWwtgQbVYLNfp2lFtV7Jt1Tn7fswdU_w9U57M4LOj_d4GinM2suRXElDJgn77D93FOYXyuwNVllQGYaHwSLkUc07UmzH5waY_BsEcejDHHkzpwRx6MLo4X1-T53ag7s34u_gCyCOQy1PYUPo3-v3UF9gqkxs</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Li, Yaoyao</creator><creator>Min, Ling</creator><creator>Zhang, Lin</creator><creator>Hu, Qin</creator><creator>Wu, Yuanlong</creator><creator>Li, Jie</creator><creator>Xie, Sai</creator><creator>Ma, Yizan</creator><creator>Zhang, Xianlong</creator><creator>Zhu, Longfu</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4947-8609</orcidid></search><sort><creationdate>20180901</creationdate><title>Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther</title><author>Li, Yaoyao ; Min, Ling ; Zhang, Lin ; Hu, Qin ; Wu, Yuanlong ; Li, Jie ; Xie, Sai ; Ma, Yizan ; Zhang, Xianlong ; Zhu, Longfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-cc09484dc9a59517b7adac1e1cdc4f0fecad306faa00a03b1372cd75ce5a92bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anthers</topic><topic>Apoptosis</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Casein</topic><topic>Casein kinase I</topic><topic>Casein Kinase I - genetics</topic><topic>Casein Kinase I - metabolism</topic><topic>Cell death</topic><topic>Cotton</topic><topic>Crop production</topic><topic>Crop yield</topic><topic>Flowers - genetics</topic><topic>Flowers - physiology</topic><topic>Gene Duplication</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Genes, Plant</topic><topic>Germination - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>High temperature</topic><topic>Hot Temperature</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Phylogeny</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoters</topic><topic>Reproduction - genetics</topic><topic>Seedlings - genetics</topic><topic>Seeds - genetics</topic><topic>Temperature effects</topic><topic>Transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yaoyao</creatorcontrib><creatorcontrib>Min, Ling</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Wu, Yuanlong</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Xie, Sai</creatorcontrib><creatorcontrib>Ma, Yizan</creatorcontrib><creatorcontrib>Zhang, Xianlong</creatorcontrib><creatorcontrib>Zhu, Longfu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yaoyao</au><au>Min, Ling</au><au>Zhang, Lin</au><au>Hu, Qin</au><au>Wu, Yuanlong</au><au>Li, Jie</au><au>Xie, Sai</au><au>Ma, Yizan</au><au>Zhang, Xianlong</au><au>Zhu, Longfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>98</volume><issue>1-2</issue><spage>33</spage><epage>49</epage><pages>33-49</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Key message
(1) We systematically analyze the promoter activities of
AtCKLs
in various tissues; (2)
AtCKL2
and
AtCKL7
were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of
AtCKL2
and
AtCKL7
expression under HT.
High temperature (HT) can seriously impede plant growth and development, causing severe loss of crop production. In
Arabidopsis, AtCKL
genes show high similarity to
GhCKI
, a gene reported to disrupt tapetal programmed cell death in cotton. However, most of
AtCKL
genes are not well characterized. Here, we systematically analyzed the expression patterns of
AtCKLs
in various tissues. The expression of
AtCKL2
and
AtCKL7
was induced in early anther development under HT, which is similar to the case of
GhCKI
. In silico analysis of
AtCKL2
and
AtCKL7
promoters indicated that four types of transcription factors (TFs) (MADS, NAC, WRKY and R2R3-MYB) might bind to
AtCKL2
and
AtCKL7
promoters. Furthermore, three MADS, three NAC, one WRKY, and three R2R3-MYB TFs were up-regulated in stage 1–8 anthers and three R2R3-MYB TFs were up-regulated in stage 9–10 anthers under HT, implying the important roles of R2R3-MYB genes in the response of anthers to HT. Among the R2R3-MYB genes,
AtMYB24
showed the similar expression as
AtCKL2
and
AtCKL7
in the anthers under HT. Additionally, yeast one-hybrid and dual-luciferase reporter system assays verified that AtMYB24 could bind to
AtCKL2
and
AtCKL7
promoters and activate the expression of these two genes. In brief, this study provides the overall expression profiles of
AtCKLs
, useful information for unraveling the molecular mechanism of
AtCKL2
and
AtCKL7
gene expression in early anther development under HT, and important clues for elucidating the mechanism of transcriptional regulation of
CKI
genes in plant anther under HT, which are critical to the reduction of crop yield loss resulting from HT.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30145767</pmid><doi>10.1007/s11103-018-0760-7</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-4947-8609</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 2018-09, Vol.98 (1-2), p.33-49 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2094420132 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Anthers Apoptosis Arabidopsis Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biochemistry Biomedical and Life Sciences Casein Casein kinase I Casein Kinase I - genetics Casein Kinase I - metabolism Cell death Cotton Crop production Crop yield Flowers - genetics Flowers - physiology Gene Duplication Gene expression Gene Expression Regulation, Plant Gene regulation Genes, Plant Germination - genetics Glucuronidase - metabolism High temperature Hot Temperature Kinases Life Sciences Phylogeny Plant growth Plant Pathology Plant Sciences Promoter Regions, Genetic - genetics Promoters Reproduction - genetics Seedlings - genetics Seeds - genetics Temperature effects Transcription factors Transcription Factors - metabolism Yeasts |
| title | Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T12%3A47%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Promoters%20of%20Arabidopsis%20Casein%20kinase%20I-like%202%20and%207%20confer%20specific%20high-temperature%20response%20in%20anther&rft.jtitle=Plant%20molecular%20biology&rft.au=Li,%20Yaoyao&rft.date=2018-09-01&rft.volume=98&rft.issue=1-2&rft.spage=33&rft.epage=49&rft.pages=33-49&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1007/s11103-018-0760-7&rft_dat=%3Cproquest_cross%3E2094420132%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2093013061&rft_id=info:pmid/30145767&rfr_iscdi=true |