Promoters of Arabidopsis Casein kinase I-like 2 and 7 confer specific high-temperature response in anther
Key message (1) We systematically analyze the promoter activities of AtCKLs in various tissues; (2) AtCKL2 and AtCKL7 were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of AtCKL2 and AtCKL7 expression under HT. High...
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Veröffentlicht in: | Plant molecular biology 2018-09, Vol.98 (1-2), p.33-49 |
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(1) We systematically analyze the promoter activities of
AtCKLs
in various tissues; (2)
AtCKL2
and
AtCKL7
were expressed in early developmental anthers under high temperature (HT) conditions; (3) AtMYB24 may function as a positive regulator of
AtCKL2
and
AtCKL7
expression under HT.
High temperature (HT) can seriously impede plant growth and development, causing severe loss of crop production. In
Arabidopsis, AtCKL
genes show high similarity to
GhCKI
, a gene reported to disrupt tapetal programmed cell death in cotton. However, most of
AtCKL
genes are not well characterized. Here, we systematically analyzed the expression patterns of
AtCKLs
in various tissues. The expression of
AtCKL2
and
AtCKL7
was induced in early anther development under HT, which is similar to the case of
GhCKI
. In silico analysis of
AtCKL2
and
AtCKL7
promoters indicated that four types of transcription factors (TFs) (MADS, NAC, WRKY and R2R3-MYB) might bind to
AtCKL2
and
AtCKL7
promoters. Furthermore, three MADS, three NAC, one WRKY, and three R2R3-MYB TFs were up-regulated in stage 1–8 anthers and three R2R3-MYB TFs were up-regulated in stage 9–10 anthers under HT, implying the important roles of R2R3-MYB genes in the response of anthers to HT. Among the R2R3-MYB genes,
AtMYB24
showed the similar expression as
AtCKL2
and
AtCKL7
in the anthers under HT. Additionally, yeast one-hybrid and dual-luciferase reporter system assays verified that AtMYB24 could bind to
AtCKL2
and
AtCKL7
promoters and activate the expression of these two genes. In brief, this study provides the overall expression profiles of
AtCKLs
, useful information for unraveling the molecular mechanism of
AtCKL2
and
AtCKL7
gene expression in early anther development under HT, and important clues for elucidating the mechanism of transcriptional regulation of
CKI
genes in plant anther under HT, which are critical to the reduction of crop yield loss resulting from HT. |
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ISSN: | 0167-4412 1573-5028 |
DOI: | 10.1007/s11103-018-0760-7 |