Effects of dietary supplementation of DL-2-hydroxy-4(methylthio) butanoic acid on antioxidant capacity and its related gene expression in lung and liver of broilers exposed to low temperature

ABSTRACT DL-2-hydroxy-4(methylthio) butanoic acid (DL-HMTBA) exhibits a higher antioxidant capability in vitro as compared to DL-Met, but the mechanism is still not known. A total of 400 8-day-old broiler chicks were allotted to a 2 [low (12 to 14°C) vs. control temperature (thermoneutral, 24 to 26°C)] × 2 (0.17% or 0.51% of DL-HMTBA) factorial arrangement to investigate effects of DL-HMTBA on antioxidant capacity and its related gene expression in lung and liver of broilers exposed to low temperature. The hepatic glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were decreased, whereas protein carbonyl and malodndialdehyde contents in lung were increased in low temperature (P < 0.05). Dietary supplementation of 0.51% DL-HMTBA increased (P < 0.05) GSH and GSH-Px activity in liver and SOD activity in lung in spite of the temperature. The greater gene expression of GSH reductase and lower expression of GSH synthetase (P < 0.01) were observed in lung of broilers that were maintained at low temperature. Higher DL-HMTBA supplementation induced greater (P < 0.05) mRNA expression of glutathione-S transferase in lung, GSH synthetase in liver and lung, as well as lower expression of GSH reductase in lung, and this effect were more obvious for the chicks exposed to low temperature (interaction, P < 0.05). Chicks that were maintained under low temperature had the lower expression of cystathionine β-synthase (CβS), whereas those fed 0.51% DL-HMTBA increased the CβS expression in liver under low temperature (interaction, P ≤ 0.05). The γ-glutamylcysteine synthetase and Met adenosyltransferase 1 (MAT1) gene expression were downregulated (P < 0.05) by low temperature and the higher (P = 0.02) expression of MAT1 was observed in lung of chicks fed 0.51% DL-HMTBA. It indicated that under low temperature, DL-HMTBA supplementation at 0.51% upregulated gene expression of GSH synthesis and Met transsulfuration pathway in liver and lung to increase the antioxidant capacity, and then mitigate the negative effects of cold stress for broilers.