Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora
Objective The objective of this study was to identify the therapeutic effect and the underlying mechanism of glucagon‐like peptide 1 (GLP‐1) in the treatment of STZ‐induced diabetes mellitus (DM). Methods Mice were treated with STZ to establish an animal model of DM, which was further treated with a...
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| Veröffentlicht in: | Journal of cellular biochemistry 2018-12, Vol.119 (12), p.10067-10074 |
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| creator | Yuan, Xiao Ni, Haixiang Chen, Xialiang Feng, Xiaohong Wu, Qiaomin Chen, Jie |
| description | Objective
The objective of this study was to identify the therapeutic effect and the underlying mechanism of glucagon‐like peptide 1 (GLP‐1) in the treatment of STZ‐induced diabetes mellitus (DM).
Methods
Mice were treated with STZ to establish an animal model of DM, which was further treated with a GLP‐1 receptor agonist. Subsequently, the status of glucose, insulin, nitric oxide, inflammatory and oxidative factors was evaluated and compared among Sham, STZ, and STZ + GLP‐1 groups. In addition, the intestinal flora spectrum in each group was also evaluated.
Results
In this study, it was found that the administration of STZ increased the level of glucose and glycosylated hemoglobin but reduced the level of insulin. It was also found that the levels of inflammation and oxidative stress in STZ‐induced DM were both enhanced, as evidenced by a decreased level of catalase, superoxide dismutase, glutathione peroxidase, as well as increased levels of malonyldialdehyde, interleukin‐1β (IL‐1β), and IL‐6. Meanwhile, the expression of nitric oxide, a factor associated with both oxidative stress and inflammation, was also suppressed in STZ‐induced DM. More importantly, the imbalance of intestinal flora was observed in STZ‐induced DM, as shown by a decreased level of both total bacteria and that of some strains including Clostridium, Bacteroides, Lactobacilli, and Bifidobacteria.
Conclusion
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora. |
| doi_str_mv | 10.1002/jcb.27343 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2091240763</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2129471058</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4193-1295a127bc31606ad2e89acdf45eee5936440ad44d9e296eb44c80943d02782d3</originalsourceid><addsrcrecordid>eNp1kc1u1DAUhS1ERaeFBS-ALLGhi7TXP0nGSxhRaFWJBWXDJnLsm8FD4gTbEZpdH4Hn4LF4EpxOYYHEypL1nU_HPoQ8Z3DOAPjFzrTnvBZSPCIrBqouZCXlY7KCWkDBBePH5CTGHQAoJfgTciyAcQWlWpGfVxZ9cp0zOrnR07Gj6QsGPeGcnKHYdWjScrvtZ6O3o_9196N3X5FOOCVnkTLq_BKhKaBOQ5Yt9Mfbzxl03s4GLbVOt5gw0gH73qU5LpmgU6TtngaMaQzOb-8tre61N7g4nM-R5LzuadePQT8lR53uIz57OE_Jp8u3t5v3xc2Hd1eb1zeFkUyJIr-s1IzXrRGsgkpbjmulje1kiYilEvlvQFsprUKuKmylNGtQUljg9ZpbcUpeHbxTGL_NuUIzuGhyc-1xnGPDQTEuoa5ERl_-g-7GOeTGmco9ZM2gXGfq7ECZMMYYsGum4AYd9g2DZhmwyQM29wNm9sWDcW4HtH_JP4tl4OIAfHc97v9vaq43bw7K36u7qCs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2129471058</pqid></control><display><type>article</type><title>Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Yuan, Xiao ; Ni, Haixiang ; Chen, Xialiang ; Feng, Xiaohong ; Wu, Qiaomin ; Chen, Jie</creator><creatorcontrib>Yuan, Xiao ; Ni, Haixiang ; Chen, Xialiang ; Feng, Xiaohong ; Wu, Qiaomin ; Chen, Jie</creatorcontrib><description>Objective
The objective of this study was to identify the therapeutic effect and the underlying mechanism of glucagon‐like peptide 1 (GLP‐1) in the treatment of STZ‐induced diabetes mellitus (DM).
Methods
Mice were treated with STZ to establish an animal model of DM, which was further treated with a GLP‐1 receptor agonist. Subsequently, the status of glucose, insulin, nitric oxide, inflammatory and oxidative factors was evaluated and compared among Sham, STZ, and STZ + GLP‐1 groups. In addition, the intestinal flora spectrum in each group was also evaluated.
Results
In this study, it was found that the administration of STZ increased the level of glucose and glycosylated hemoglobin but reduced the level of insulin. It was also found that the levels of inflammation and oxidative stress in STZ‐induced DM were both enhanced, as evidenced by a decreased level of catalase, superoxide dismutase, glutathione peroxidase, as well as increased levels of malonyldialdehyde, interleukin‐1β (IL‐1β), and IL‐6. Meanwhile, the expression of nitric oxide, a factor associated with both oxidative stress and inflammation, was also suppressed in STZ‐induced DM. More importantly, the imbalance of intestinal flora was observed in STZ‐induced DM, as shown by a decreased level of both total bacteria and that of some strains including Clostridium, Bacteroides, Lactobacilli, and Bifidobacteria.
Conclusion
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.27343</identifier><identifier>PMID: 30129059</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animal models ; blood glucose ; Catalase ; Diabetes ; Diabetes mellitus ; Flora ; GLP-1 receptor agonists ; GLP‐1 ; Glucagon ; Glucose ; Glutathione ; Glutathione peroxidase ; Hemoglobin ; Inflammation ; inflammatory status ; Insulin ; Interleukins ; intestinal flora imbalance ; Intestinal microflora ; Intestine ; Lactobacilli ; Nitric oxide ; oxidative status ; Oxidative stress ; Peptides ; Peroxidase ; Superoxide dismutase</subject><ispartof>Journal of cellular biochemistry, 2018-12, Vol.119 (12), p.10067-10074</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4193-1295a127bc31606ad2e89acdf45eee5936440ad44d9e296eb44c80943d02782d3</citedby><cites>FETCH-LOGICAL-c4193-1295a127bc31606ad2e89acdf45eee5936440ad44d9e296eb44c80943d02782d3</cites><orcidid>0000-0001-9239-3734</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.27343$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.27343$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30129059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Xiao</creatorcontrib><creatorcontrib>Ni, Haixiang</creatorcontrib><creatorcontrib>Chen, Xialiang</creatorcontrib><creatorcontrib>Feng, Xiaohong</creatorcontrib><creatorcontrib>Wu, Qiaomin</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><title>Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Objective
The objective of this study was to identify the therapeutic effect and the underlying mechanism of glucagon‐like peptide 1 (GLP‐1) in the treatment of STZ‐induced diabetes mellitus (DM).
Methods
Mice were treated with STZ to establish an animal model of DM, which was further treated with a GLP‐1 receptor agonist. Subsequently, the status of glucose, insulin, nitric oxide, inflammatory and oxidative factors was evaluated and compared among Sham, STZ, and STZ + GLP‐1 groups. In addition, the intestinal flora spectrum in each group was also evaluated.
Results
In this study, it was found that the administration of STZ increased the level of glucose and glycosylated hemoglobin but reduced the level of insulin. It was also found that the levels of inflammation and oxidative stress in STZ‐induced DM were both enhanced, as evidenced by a decreased level of catalase, superoxide dismutase, glutathione peroxidase, as well as increased levels of malonyldialdehyde, interleukin‐1β (IL‐1β), and IL‐6. Meanwhile, the expression of nitric oxide, a factor associated with both oxidative stress and inflammation, was also suppressed in STZ‐induced DM. More importantly, the imbalance of intestinal flora was observed in STZ‐induced DM, as shown by a decreased level of both total bacteria and that of some strains including Clostridium, Bacteroides, Lactobacilli, and Bifidobacteria.
Conclusion
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.</description><subject>Animal models</subject><subject>blood glucose</subject><subject>Catalase</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Flora</subject><subject>GLP-1 receptor agonists</subject><subject>GLP‐1</subject><subject>Glucagon</subject><subject>Glucose</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Hemoglobin</subject><subject>Inflammation</subject><subject>inflammatory status</subject><subject>Insulin</subject><subject>Interleukins</subject><subject>intestinal flora imbalance</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Lactobacilli</subject><subject>Nitric oxide</subject><subject>oxidative status</subject><subject>Oxidative stress</subject><subject>Peptides</subject><subject>Peroxidase</subject><subject>Superoxide dismutase</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAUhS1ERaeFBS-ALLGhi7TXP0nGSxhRaFWJBWXDJnLsm8FD4gTbEZpdH4Hn4LF4EpxOYYHEypL1nU_HPoQ8Z3DOAPjFzrTnvBZSPCIrBqouZCXlY7KCWkDBBePH5CTGHQAoJfgTciyAcQWlWpGfVxZ9cp0zOrnR07Gj6QsGPeGcnKHYdWjScrvtZ6O3o_9196N3X5FOOCVnkTLq_BKhKaBOQ5Yt9Mfbzxl03s4GLbVOt5gw0gH73qU5LpmgU6TtngaMaQzOb-8tre61N7g4nM-R5LzuadePQT8lR53uIz57OE_Jp8u3t5v3xc2Hd1eb1zeFkUyJIr-s1IzXrRGsgkpbjmulje1kiYilEvlvQFsprUKuKmylNGtQUljg9ZpbcUpeHbxTGL_NuUIzuGhyc-1xnGPDQTEuoa5ERl_-g-7GOeTGmco9ZM2gXGfq7ECZMMYYsGum4AYd9g2DZhmwyQM29wNm9sWDcW4HtH_JP4tl4OIAfHc97v9vaq43bw7K36u7qCs</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Yuan, Xiao</creator><creator>Ni, Haixiang</creator><creator>Chen, Xialiang</creator><creator>Feng, Xiaohong</creator><creator>Wu, Qiaomin</creator><creator>Chen, Jie</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9239-3734</orcidid></search><sort><creationdate>201812</creationdate><title>Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora</title><author>Yuan, Xiao ; Ni, Haixiang ; Chen, Xialiang ; Feng, Xiaohong ; Wu, Qiaomin ; Chen, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4193-1295a127bc31606ad2e89acdf45eee5936440ad44d9e296eb44c80943d02782d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal models</topic><topic>blood glucose</topic><topic>Catalase</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Flora</topic><topic>GLP-1 receptor agonists</topic><topic>GLP‐1</topic><topic>Glucagon</topic><topic>Glucose</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Hemoglobin</topic><topic>Inflammation</topic><topic>inflammatory status</topic><topic>Insulin</topic><topic>Interleukins</topic><topic>intestinal flora imbalance</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Lactobacilli</topic><topic>Nitric oxide</topic><topic>oxidative status</topic><topic>Oxidative stress</topic><topic>Peptides</topic><topic>Peroxidase</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Xiao</creatorcontrib><creatorcontrib>Ni, Haixiang</creatorcontrib><creatorcontrib>Chen, Xialiang</creatorcontrib><creatorcontrib>Feng, Xiaohong</creatorcontrib><creatorcontrib>Wu, Qiaomin</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Xiao</au><au>Ni, Haixiang</au><au>Chen, Xialiang</au><au>Feng, Xiaohong</au><au>Wu, Qiaomin</au><au>Chen, Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2018-12</date><risdate>2018</risdate><volume>119</volume><issue>12</issue><spage>10067</spage><epage>10074</epage><pages>10067-10074</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Objective
The objective of this study was to identify the therapeutic effect and the underlying mechanism of glucagon‐like peptide 1 (GLP‐1) in the treatment of STZ‐induced diabetes mellitus (DM).
Methods
Mice were treated with STZ to establish an animal model of DM, which was further treated with a GLP‐1 receptor agonist. Subsequently, the status of glucose, insulin, nitric oxide, inflammatory and oxidative factors was evaluated and compared among Sham, STZ, and STZ + GLP‐1 groups. In addition, the intestinal flora spectrum in each group was also evaluated.
Results
In this study, it was found that the administration of STZ increased the level of glucose and glycosylated hemoglobin but reduced the level of insulin. It was also found that the levels of inflammation and oxidative stress in STZ‐induced DM were both enhanced, as evidenced by a decreased level of catalase, superoxide dismutase, glutathione peroxidase, as well as increased levels of malonyldialdehyde, interleukin‐1β (IL‐1β), and IL‐6. Meanwhile, the expression of nitric oxide, a factor associated with both oxidative stress and inflammation, was also suppressed in STZ‐induced DM. More importantly, the imbalance of intestinal flora was observed in STZ‐induced DM, as shown by a decreased level of both total bacteria and that of some strains including Clostridium, Bacteroides, Lactobacilli, and Bifidobacteria.
Conclusion
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.
In summary, the findings of this study confirmed the antihyperglycemic effect of GLP‐1 and demonstrated that the therapeutic effect of GLP‐1 in the treatment of STZ‐induced DM was mediated, at least partially, by its ability to restore the balance of intestinal flora.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30129059</pmid><doi>10.1002/jcb.27343</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9239-3734</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Animal models blood glucose Catalase Diabetes Diabetes mellitus Flora GLP-1 receptor agonists GLP‐1 Glucagon Glucose Glutathione Glutathione peroxidase Hemoglobin Inflammation inflammatory status Insulin Interleukins intestinal flora imbalance Intestinal microflora Intestine Lactobacilli Nitric oxide oxidative status Oxidative stress Peptides Peroxidase Superoxide dismutase |
| title | Identification of therapeutic effect of glucagon‐like peptide 1 in the treatment of STZ‐induced diabetes mellitus in rats by restoring the balance of intestinal flora |
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