Fabrication of PCL/PVP Electrospun Fibers loaded with Trans-anethole for Bone Regeneration in vitro

[Display omitted] •Fabrication of trans-Anethole loaded PCL/PVP fibers by electrospinning technique.•Characterization by physiochemical, material and biological studies.•Induction of osteogenesis by trans-Anethole.•Regulation of Runx2 co-activators by trans-Anethole promoting bone regeneration. Trans-anethole (TA) is a phenolic phytocompound widely used in the food and health sector because of its diverse biological properties. However, its role in the promotion of bone formation is not known. With the enhanced bioavailability of TA, we aimed to determine its effect on osteogenesis; TA at different concentrations (5, 10, and 20 μM) was loaded onto polycaprolactone (PCL)/polyvinylpyrrolidone (PVP) fibers by the electrospinning technique. The synthesized PCL/PVP + TA fibers were subjected to physiochemical and material characterization. The addition of TA did not have any effect on fiber thickness, swelling, protein adsorption, degradation, or biomineralization. The fibers were compatible with mouse mesenchymal stem cells (mMSCs). A sustained release of TA from the fibers promoted osteoblast differentiation at the cellular and molecular levels. Furthermore, the release of TA from fibers up-regulated the expression of Runx2, a bone transcription factor, and its co-activators, which are key molecules for osteoblast differentiation. Thus, these results provide insights into the bioavailability of TA in promoting in vitro osteoblast differentiation and the potential applications of TA in bone regeneration.