Pseudomonas aeruginosa FARP72 Offers Protection Against Aeromonas hydrophila Infection in Labeo rohita
Use of probiotics as the biocontrol agent for disease prevention in aquaculture is gaining importance as an alternative to the indiscriminate use of antibiotics and other chemotherapeutics. In view of this trend, the probiotic properties of a potent antagonistic bacterium, Pseudomonas aeruginosa FAR...
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Veröffentlicht in: | Probiotics and antimicrobial proteins 2019-09, Vol.11 (3), p.973-980 |
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Sprache: | eng |
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Zusammenfassung: | Use of probiotics as the biocontrol agent for disease prevention in aquaculture is gaining importance as an alternative to the indiscriminate use of antibiotics and other chemotherapeutics. In view of this trend, the probiotic properties of a potent antagonistic bacterium,
Pseudomonas aeruginosa
FARP
72
, was characterized in terms of safety, antagonistic activities, in vitro immunomodulation, and in vivo disease resistance. Immunomodulatory activity was ascertained by measuring the production of intracellular superoxide anion, nitric oxide, total leukocyte peroxidase content, and the leukocyte proliferation in head kidney leukocytes. The bacterium isolated from the skin mucus of freshwater catfish
Clarias batrachus
was harmless to
Labeo rohita
. It showed inhibitory activity against
Aeromonas caviae
,
A. hydrophila
,
Edwardsiella tarda
,
Pseudomonas putida
, and
Streptococcus agalactiae
as revealed by cross and parallel streaking methods. Significantly higher superoxide anion and nitric oxide production, peroxidase content, and proliferative responses of leucocytes delineated the strains’ ability to interact with immune cells to activate the immune system in vitro. Significant growth inhibition of
A. hydrophila
from 1.55 × 10
5
CFU/mL was observed when co-cultured with
P. aeruginosa
FARP
72
in phosphate-buffered saline (PBS) at levels ranging from 2.61 × 10
7
to 2.61 × 10
9
CFU/mL in 10 days.
P. aeruginosa
FARP
72
increased the survival rate of rohu fingerlings against pathogenic
A. hydrophila
challenge in biocontrol study in vivo as determined by cohabitation challenge. These results suggest that
P. aeruginosa
FARP
72
is a potential probiotic strain and can be used in aquaculture to improve the health status and disease resistance of fish. |
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ISSN: | 1867-1306 1867-1314 |
DOI: | 10.1007/s12602-018-9456-1 |