Phosphorescence Lifetime Imaging of Labile Zn2+ in Mitochondria via a Phosphorescent Iridium(III) Complex

Phosphorescence lifetime Zn2+ imaging possesses the advantage over normal fluorescence imaging in offering the more accurate temporal-spatial Zn2+ information. Herein, we report a new phosphorescent cyclometalated Ir­(III) complex with a Zn2+-chelator bearing 1,10-phenanthrolin acting as ancillary l...

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Veröffentlicht in:Inorganic chemistry 2018-09, Vol.57 (17), p.10625-10632
Hauptverfasser: Zhang, Changli, Liu, Minsheng, Liu, Shaoxian, Yang, Hui, Zhao, Qiang, Liu, Zhipeng, He, Weijiang
Format: Artikel
Sprache:eng
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Zusammenfassung:Phosphorescence lifetime Zn2+ imaging possesses the advantage over normal fluorescence imaging in offering the more accurate temporal-spatial Zn2+ information. Herein, we report a new phosphorescent cyclometalated Ir­(III) complex with a Zn2+-chelator bearing 1,10-phenanthrolin acting as ancillary ligand, Zin-IrDPA, which displays the specific Zn2+-induced enhancement of phosphorescence and phosphorescence lifetime, and the mitochondria-targeting ability. Moreover, its Zn2+-induced phosphorescence lifetime enhancement factor is not affected by medium lipophilicity, viscosity, polarity, and especially ambient oxygen. The reversible tracking of introduced exogenous labile Zn2+ in MCF-7 and HeLa cells via phosphorescence imaging and phosphorescence lifetime imaging (PLIM) have been realized with Zin-IrDPA. Moreover, PLIM with Zin-IrDPA is able to track the SNOC-stimulated endogenous Zn2+ release in mitochondria.
ISSN:0020-1669
1520-510X
DOI:10.1021/acs.inorgchem.8b01272