Sequential simplex optimization of recombinant biotinylated survivin production by Escherichia coli using mineral supplementation
The sequential simplex method is a useful tool for rapidly optimizing a process by moving through factor space via a relatively simple geometric algorithm. This method was employed to optimize growth and survivin-BCCP production in Escherichia coli Origami B using zinc sulphate supplemented culture...
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Veröffentlicht in: | Biochemical engineering journal 2009-10, Vol.46 (2), p.115-120 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The sequential simplex method is a useful tool for rapidly optimizing a process by moving through factor space via a relatively simple geometric algorithm. This method was employed to optimize growth and survivin-BCCP production in
Escherichia coli Origami B using zinc sulphate supplemented culture medium. Five experimental parameters were investigated: concentration of zinc sulphate and IPTG, pH, temperature, and agitation rate. Optimized conditions were as follows: 190
μM zinc sulphate with 246
μM IPTG, pH 7, at a temperature of 23.5
°C and agitation rate of 345
rpm. The results for cell density and survivin-BCCP production were, 17% and 140% higher than in non-zinc supplemented medium and 12.6% and 25.5% higher than could be obtained by varying one-factor-at-a-time. The present work demonstrates the effectiveness of zinc sulphate supplementation and the efficiency of the sequential simplex optimization method in obtaining high yields. |
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ISSN: | 1369-703X 1873-295X |
DOI: | 10.1016/j.bej.2009.04.015 |