New ectopic vestibular hair cell-like cells induced by Math1 gene transfer in postnatal rats

Abstract Overexpression of Math1 has been demonstrated to induce robust ectopic new hair cells in greater epithelial ridge (GER) and lesser epithelial ridge (LER) of the postnatal rats' cochlear in vitro. In spite of the similarities between cochlear and vestibular epithelia in origin and structure, no similar results have been reported in the non-sensory region of vestibular epithelia in vitro. In the study, the adenoviral vectors inserted with Math1 gene were constructed to examine their effect on vestibular epithelia in postnatal rats. In vivo, the adenovirus vectors administered in vestibular perilymphatic or endolymphatic space, their transduction efficiency and other indexes are different. We set up a culture construction to simulate and show the differences. In the study, we also developed a new dissection protocol to be quick to harvest the whole maculae and cristae. The new ectopic vestibular hair cell-like cells induced by overexpression of Math1, were found to appear in the non-sensory region of the postnatal rats' vestibular epithelia as observed in the cochlear, and the number of the new cells was different when a different virus administration was simulated in vestibular perilymphatic or endolymphatic space, suggesting that the cells found could have the capability to differentiate into new hair cells. Our study might pave the way for further in vivo studies on vestibular Math1 adenoviral vector gene transfer.