An evolution-based analysis scheme to identify CO sub(2)/O sub(2) specificity-determining factors for ribulose 1,5-bisphosphate carboxylase/oxygenase

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo) catalyzes a rate-limiting step in photosynthetic carbon assimilation (reacting with CO sub(2)) and its competitive photo-respiratory carbon oxidation (reacting with O sub(2)). RuBisCo enzyme with an enhanced CO sub(2)/O sub(2) specificity would boost the ability to make great progress in agricultural production and environmental management. RuBisCos in marine non-green algae, resulting from an earlier endo-symbiotic event, diverge greatly from those in green plants and cyanobacteria and, further, have the highest CO sub(2)/O sub(2) specificity whereas RuBisCos in cyanobacteria have the lowest. We assumed that there exist different levels of CO sub(2)/O sub(2) specificity-determining factors, corresponding to different evolutionary events and specificity levels. Based on this assumption, we devised a scheme to identify these substrate-determining factors. From this analysis, we are able to discover different categories of the CO sub(2)/O sub(2) specificity-determining factors that show which residue substitutions account for (relatively) small specificity changes, as happened in green plants, or a tremendous enhancement, as observed in marine non-green algae. Therefore, the analysis can improve our understanding of molecular mechanisms in the substrate specificity development and prioritize candidate specificity-determining surface residues for site-directed mutagenesis.