Negative Results of umu Genotoxicity Test of Fluorotelomer Alcohols and Perfluorinated Alkyl Acids

Objectives： Recently, perfluorooctanoate (PFOA) has been ubiquitously detected in the environment as well as in human serum. Fluorotelomer alcohols (FTOHs), a precursor of PFOA, undergo bio-degradation via several metabolic routes which leads to formation of various biodegradation products. The degradation of FTOHs produces an α,β-unsaturated aldehyde that seems possibly to be electrophilic and may react with cellular macromolecules including DNA. Methods： We investigated the genotoxicity of three FTOHs (6：2 FTOH, 8：2 FTOH and 10：2 FTOH), PFOA and perfluorooctane sulfonate (PFOS) using the umu test. Results： The FTOHs, PFOA and PFOS showed no significant increases in β-galactosidase activity at 0-1000 μM in the absence of S9 mix. The results were unchanged by the metabolic activation with S9 mix. Conclusion： The genotoxicities of FTOHs, PFOA or PFOS are not detectable using the present method, suggesting that they are unlikely mutagens.