Autocrine S100B effects on astrocytes are mediated via RAGE
Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astr...
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| Veröffentlicht in: | Journal of neuroimmunology 2007-03, Vol.184 (1), p.214-222 |
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| container_title | Journal of neuroimmunology |
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| creator | Ponath, Gerald Schettler, Christiane Kaestner, Florian Voigt, Björn Wentker, Dennis Arolt, Volker Rothermundt, Matthias |
| description | Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression. |
| doi_str_mv | 10.1016/j.jneuroim.2006.12.011 |
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The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/j.jneuroim.2006.12.011</identifier><identifier>PMID: 17254641</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergy and Immunology ; Analysis of Variance ; Animals ; Animals, Newborn ; Astrocytes - drug effects ; Astrocytes - ultrastructure ; autocrine pathway ; Blotting, Western ; Cells, Cultured ; Cerebral Cortex - cytology ; Dose-Response Relationship, Drug ; Enzyme Activation - drug effects ; Enzyme-Linked Immunosorbent Assay - methods ; Flow Cytometry ; Gene Expression - drug effects ; Glia ; Glial Fibrillary Acidic Protein - metabolism ; Interleukin-6 - metabolism ; Mice ; Mitogen-Activated Protein Kinases - metabolism ; Nerve Growth Factors - pharmacology ; Neurology ; NF-kappaB-Inducing Kinase ; proinflammatory cytokines ; Protein Serine-Threonine Kinases - metabolism ; RAGE ; S100 Calcium Binding Protein beta Subunit ; S100 Proteins - pharmacology ; S100B proteins ; Time Factors ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Journal of neuroimmunology, 2007-03, Vol.184 (1), p.214-222</ispartof><rights>Elsevier B.V.</rights><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-795b11607c7ab62121ae643349e7bfc2a7f4f62df47d06c079d5124df1b6e7153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165572806005248$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17254641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ponath, Gerald</creatorcontrib><creatorcontrib>Schettler, Christiane</creatorcontrib><creatorcontrib>Kaestner, Florian</creatorcontrib><creatorcontrib>Voigt, Björn</creatorcontrib><creatorcontrib>Wentker, Dennis</creatorcontrib><creatorcontrib>Arolt, Volker</creatorcontrib><creatorcontrib>Rothermundt, Matthias</creatorcontrib><title>Autocrine S100B effects on astrocytes are mediated via RAGE</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression.</description><subject>Allergy and Immunology</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - ultrastructure</subject><subject>autocrine pathway</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activation - drug effects</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Flow Cytometry</subject><subject>Gene Expression - drug effects</subject><subject>Glia</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Interleukin-6 - metabolism</subject><subject>Mice</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neurology</subject><subject>NF-kappaB-Inducing Kinase</subject><subject>proinflammatory cytokines</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>RAGE</subject><subject>S100 Calcium Binding Protein beta Subunit</subject><subject>S100 Proteins - pharmacology</subject><subject>S100B proteins</subject><subject>Time Factors</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpSDZp_kLwKTc7M1pZ8lII3YQkLQQCTXsWsjwCuV4rlezA_vto2S2BXnKay_POxzOMXSBUCCiv-qofaY7BbyoOICvkFSB-YgtsFC8bwfEzW2SwLmvFmxN2mlIPgPVSrI7ZCSpeCylwwb6u5ynY6EcqnhHgpiDnyE6pCGNh0hSD3U6UChOp2FDnzURd8epN8XP9cPeFHTkzJDo_1DP2-_7u1-338vHp4cft-rG0NTZTqVZ1iyhBWWVayZGjISmWeRNSrbPcKCec5J0TqgNpQa26GrnoHLaSVF75jF3u-77E8HemNOmNT5aGwYwU5qQ5KLVUqDIo96CNIaVITr9EvzFxqxH0Tpvu9T9teqdNI9dZWw5eHCbMbT7zPXbwlIFve4Dyna-eok7W02izkph16S74j2dc_9fCDn701gx_aEupD3Mcs0WNOuWAft49b_c7kAA1F83yDWENlGk</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Ponath, Gerald</creator><creator>Schettler, Christiane</creator><creator>Kaestner, Florian</creator><creator>Voigt, Björn</creator><creator>Wentker, Dennis</creator><creator>Arolt, Volker</creator><creator>Rothermundt, Matthias</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope></search><sort><creationdate>20070301</creationdate><title>Autocrine S100B effects on astrocytes are mediated via RAGE</title><author>Ponath, Gerald ; Schettler, Christiane ; Kaestner, Florian ; Voigt, Björn ; Wentker, Dennis ; Arolt, Volker ; Rothermundt, Matthias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-795b11607c7ab62121ae643349e7bfc2a7f4f62df47d06c079d5124df1b6e7153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Allergy and Immunology</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - ultrastructure</topic><topic>autocrine pathway</topic><topic>Blotting, Western</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activation - drug effects</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Flow Cytometry</topic><topic>Gene Expression - drug effects</topic><topic>Glia</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Interleukin-6 - metabolism</topic><topic>Mice</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neurology</topic><topic>NF-kappaB-Inducing Kinase</topic><topic>proinflammatory cytokines</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>RAGE</topic><topic>S100 Calcium Binding Protein beta Subunit</topic><topic>S100 Proteins - pharmacology</topic><topic>S100B proteins</topic><topic>Time Factors</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ponath, Gerald</creatorcontrib><creatorcontrib>Schettler, Christiane</creatorcontrib><creatorcontrib>Kaestner, Florian</creatorcontrib><creatorcontrib>Voigt, Björn</creatorcontrib><creatorcontrib>Wentker, Dennis</creatorcontrib><creatorcontrib>Arolt, Volker</creatorcontrib><creatorcontrib>Rothermundt, Matthias</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ponath, Gerald</au><au>Schettler, Christiane</au><au>Kaestner, Florian</au><au>Voigt, Björn</au><au>Wentker, Dennis</au><au>Arolt, Volker</au><au>Rothermundt, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autocrine S100B effects on astrocytes are mediated via RAGE</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>184</volume><issue>1</issue><spage>214</spage><epage>222</epage><pages>214-222</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17254641</pmid><doi>10.1016/j.jneuroim.2006.12.011</doi><tpages>9</tpages></addata></record> |
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| subjects | Allergy and Immunology Analysis of Variance Animals Animals, Newborn Astrocytes - drug effects Astrocytes - ultrastructure autocrine pathway Blotting, Western Cells, Cultured Cerebral Cortex - cytology Dose-Response Relationship, Drug Enzyme Activation - drug effects Enzyme-Linked Immunosorbent Assay - methods Flow Cytometry Gene Expression - drug effects Glia Glial Fibrillary Acidic Protein - metabolism Interleukin-6 - metabolism Mice Mitogen-Activated Protein Kinases - metabolism Nerve Growth Factors - pharmacology Neurology NF-kappaB-Inducing Kinase proinflammatory cytokines Protein Serine-Threonine Kinases - metabolism RAGE S100 Calcium Binding Protein beta Subunit S100 Proteins - pharmacology S100B proteins Time Factors Tumor Necrosis Factor-alpha - metabolism |
| title | Autocrine S100B effects on astrocytes are mediated via RAGE |
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