Autocrine S100B effects on astrocytes are mediated via RAGE

Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of neuroimmunology 2007-03, Vol.184 (1), p.214-222
Hauptverfasser:	Ponath, Gerald, Schettler, Christiane, Kaestner, Florian, Voigt, Björn, Wentker, Dennis, Arolt, Volker, Rothermundt, Matthias
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Analysis of Variance Animals Animals, Newborn Astrocytes - drug effects Astrocytes - ultrastructure autocrine pathway Blotting, Western Cells, Cultured Cerebral Cortex - cytology Dose-Response Relationship, Drug Enzyme Activation - drug effects Enzyme-Linked Immunosorbent Assay - methods Flow Cytometry Gene Expression - drug effects Glia Glial Fibrillary Acidic Protein - metabolism Interleukin-6 - metabolism Mice Mitogen-Activated Protein Kinases - metabolism Nerve Growth Factors - pharmacology Neurology NF-kappaB-Inducing Kinase proinflammatory cytokines Protein Serine-Threonine Kinases - metabolism RAGE S100 Calcium Binding Protein beta Subunit S100 Proteins - pharmacology S100B proteins Time Factors Tumor Necrosis Factor-alpha - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Abstract To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression.
ISSN:	0165-5728 1872-8421
DOI:	10.1016/j.jneuroim.2006.12.011