Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: Structure and potential prebiotic activity
Cultivated oyster mushrooms (genus Pleurotus) are interesting as a source of biologically active glucans. Partially, β-glucan from Pleurotus sp. (pleuran) has been used as food supplements due to its immunosuppressive activity. Like other dietary fibre components, oyster mushroom polysaccharides can...
Gespeichert in:
Veröffentlicht in: | Carbohydrate polymers 2009-05, Vol.76 (4), p.548-556 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Cultivated oyster mushrooms (genus
Pleurotus) are interesting as a source of biologically active glucans. Partially, β-glucan from
Pleurotus sp. (pleuran) has been used as food supplements due to its immunosuppressive activity. Like other dietary fibre components, oyster mushroom polysaccharides can stimulate the growth of colon microorganisms (probiotics), i.e. act as prebiotics. Specific glucans were isolated from stems of
Pleurotus ostreatus and
Pleurotus eryngii by subsequent boiling water and alkali extraction. Obtained water soluble (
L1), alkali soluble (
L2) and insoluble (
S) fractions were characterised by various analytical methods. Spectroscopic analysis detected glucans in all the fractions: branched 1,3-1,6-β-
d-glucan predominated in
L1 and
S, while linear 1,3-α-
d-glucan in
L2. Fractions
L1 also contained marked amount of proteins partially in complex with glucans; protein content in
L2 was insignificant. Effective deproteinisation of
L1 and separation of α- and β-glucans in
L2 was achieved by the treatment with phenolic reagent. Small amount of chitin was found in
S as a component of cell wall chitin–glucan complex. Potential prebiotic activity of extracts
L1 and
L2 was testing using nine probiotic strains of
Lactobacillus,
Bifidobacterium and
Enterococcus. These probiotics showed different growth characteristics dependently on used extract and strain specificity due to the presence of structurally diverse compounds. The extracts
L1 and
L2 can be applied to synbiotic construction only for carefully selected probiotic strains. This exploitation of fruit body extracts extends the use of mushrooms
P. ostreatus and
P. eryngii for human health. |
---|---|
ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2008.11.021 |