Nuclear penetration of surface functionalized gold nanoparticles
Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptop...
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| Veröffentlicht in: | Toxicology and applied pharmacology 2009-06, Vol.237 (2), p.196-204 |
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| creator | Gu, Yan-Juan Cheng, Jinping Lin, Chun-Chi Lam, Yun Wah Cheng, Shuk Han Wong, Wing-Tak |
| description | Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH
2-PEG-NH
2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier. |
| doi_str_mv | 10.1016/j.taap.2009.03.009 |
| format | Article |
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2-PEG-NH
2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1016/j.taap.2009.03.009</identifier><identifier>PMID: 19328820</identifier><identifier>CODEN: TXAPA9</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biomaterials ; Cell Nucleus - metabolism ; Cell Survival - drug effects ; Chemical and industrial products toxicology. Toxic occupational diseases ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Fluorescein-5-isothiocyanate ; Gold - chemistry ; Gold - toxicity ; Gold nanoparticles ; HeLa Cells ; Humans ; Medical sciences ; Metals and various inorganic compounds ; Molecular Structure ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Nanoparticles - ultrastructure ; Nuclear penetration ; Polyethylene Glycols - chemistry ; Polyethylene Glycols - toxicity ; Surface modification ; Toxicology</subject><ispartof>Toxicology and applied pharmacology, 2009-06, Vol.237 (2), p.196-204</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-d2ff469afc7a0a9394c81a694325700fe87f261d1b905cfba4a9817714f3cab93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0041008X09001203$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21514863$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19328820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Yan-Juan</creatorcontrib><creatorcontrib>Cheng, Jinping</creatorcontrib><creatorcontrib>Lin, Chun-Chi</creatorcontrib><creatorcontrib>Lam, Yun Wah</creatorcontrib><creatorcontrib>Cheng, Shuk Han</creatorcontrib><creatorcontrib>Wong, Wing-Tak</creatorcontrib><title>Nuclear penetration of surface functionalized gold nanoparticles</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH
2-PEG-NH
2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier.</description><subject>Biological and medical sciences</subject><subject>Biomaterials</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Administration Schedule</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Gold - chemistry</subject><subject>Gold - toxicity</subject><subject>Gold nanoparticles</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Molecular Structure</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nuclear penetration</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polyethylene Glycols - toxicity</subject><subject>Surface modification</subject><subject>Toxicology</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr20AUhYfQELtu_0AWRZtmJ-XOQ4-BLFpMmhZMskmgu-F6dCeMkSV1Rgqkv75jbNJdVgcu3zlcPsYuORQceHW9KybEsRAAugBZpDhjSw66ykFK-YEtARTPAZrfC_Yxxh0kQil-wRZcS9E0Apbs2_1sO8KQjdTTFHDyQ58NLotzcGgpc3NvDzfs_F9qs-eha7Me-2HEMPnUjJ_YucMu0udTrtjTj9vH9c9883D3a_19k1vV8ClvhXOq0uhsjYBaamUbjpVWUpQ1gKOmdqLiLd9qKK3bokLd8LrmykmLWy1X7Oq4O4bhz0xxMnsfLXUd9jTM0QioSyihSqA4gjYMMQZyZgx-j-HVcDAHb2ZnDt7MwZsBaVKk0pfT-rzdU_u_chKVgK8nAKPFzgXsrY9vnOAlV00lE3dz5Ci5ePEUTLSeekutD2Qn0w7-vT_-ARq2i-I</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Gu, Yan-Juan</creator><creator>Cheng, Jinping</creator><creator>Lin, Chun-Chi</creator><creator>Lam, Yun Wah</creator><creator>Cheng, Shuk Han</creator><creator>Wong, Wing-Tak</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090601</creationdate><title>Nuclear penetration of surface functionalized gold nanoparticles</title><author>Gu, Yan-Juan ; Cheng, Jinping ; Lin, Chun-Chi ; Lam, Yun Wah ; Cheng, Shuk Han ; Wong, Wing-Tak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-d2ff469afc7a0a9394c81a694325700fe87f261d1b905cfba4a9817714f3cab93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Biomaterials</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Administration Schedule</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Gold - chemistry</topic><topic>Gold - toxicity</topic><topic>Gold nanoparticles</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Molecular Structure</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - toxicity</topic><topic>Nanoparticles - ultrastructure</topic><topic>Nuclear penetration</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polyethylene Glycols - toxicity</topic><topic>Surface modification</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Yan-Juan</creatorcontrib><creatorcontrib>Cheng, Jinping</creatorcontrib><creatorcontrib>Lin, Chun-Chi</creatorcontrib><creatorcontrib>Lam, Yun Wah</creatorcontrib><creatorcontrib>Cheng, Shuk Han</creatorcontrib><creatorcontrib>Wong, Wing-Tak</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Yan-Juan</au><au>Cheng, Jinping</au><au>Lin, Chun-Chi</au><au>Lam, Yun Wah</au><au>Cheng, Shuk Han</au><au>Wong, Wing-Tak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear penetration of surface functionalized gold nanoparticles</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>237</volume><issue>2</issue><spage>196</spage><epage>204</epage><pages>196-204</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><coden>TXAPA9</coden><abstract>Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH
2-PEG-NH
2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>19328820</pmid><doi>10.1016/j.taap.2009.03.009</doi><tpages>9</tpages></addata></record> |
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| subjects | Biological and medical sciences Biomaterials Cell Nucleus - metabolism Cell Survival - drug effects Chemical and industrial products toxicology. Toxic occupational diseases Dose-Response Relationship, Drug Drug Administration Schedule Fluorescein-5-isothiocyanate Gold - chemistry Gold - toxicity Gold nanoparticles HeLa Cells Humans Medical sciences Metals and various inorganic compounds Molecular Structure Nanoparticles - chemistry Nanoparticles - toxicity Nanoparticles - ultrastructure Nuclear penetration Polyethylene Glycols - chemistry Polyethylene Glycols - toxicity Surface modification Toxicology |
| title | Nuclear penetration of surface functionalized gold nanoparticles |
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