Chitosan as a growth stimulator in orchid tissue culture
The effect of shrimp and fungal chitosan on the growth and development of orchid plant meristemic tissue in culture was investigated in liquid and on solid medium. The growth of meristem explants into protocorm-like bodies in liquid medium was accelerated up to 15 times in the presence of chitosan o...
Gespeichert in:
Veröffentlicht in: | Plant science (Limerick) 2006-06, Vol.170 (6), p.1185-1190 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The effect of shrimp and fungal chitosan on the growth and development of orchid plant meristemic tissue in culture was investigated in liquid and on solid medium. The growth of meristem explants into protocorm-like bodies in liquid medium was accelerated up to 15 times in the presence of chitosan oligomer, the optimal concentration being 15
ppm. The 1
kDa shrimp oligomer was slightly more effective compared to 10
kDa shrimp chitosan and four times more active compared to high molecular weight 100
kDa shrimp chitosan. The 10
kDa fungal chitosan was more effective compared with 1
kDa oligomer. The development of orchid protocorm into differentiated orchid tissue with primary shoots and roots was studied on solid agar medium. The optimal effect, the generation of 5–7 plantlets in 12 weeks was observed in the presence of 20
ppm using either 10
kDa fungal or 1
kDa oligomer shrimp chitosan. The data are consistent with preliminary results from field experiments and confirm unequivocally that a minor amount of chitosan has a profound effect on the growth and development of orchid plant tissue. |
---|---|
ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/j.plantsci.2006.02.006 |