Effects of Targeting lncRNA HOTAIR on the Invasion and Nude Mouse Tumorigenicity of Epithelial Ovarian Cancer Cells
To explore the effects of shRNA-mediated downregulating lncRNA on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells. The expression of lncRNA was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA gene was cloned into RNA interference...
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| Veröffentlicht in: | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2018-05, Vol.49 (3), p.352-357 |
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| container_title | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban |
| container_volume | 49 |
| creator | Zhou, Yu-Fu Chen, Deng-Yu Chu, Yi-Fan Zheng, Qing-Wei Xu, Zhi-Ben |
| description | To explore the effects of shRNA-mediated downregulating lncRNA
on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells.
The expression of lncRNA
was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA
gene was cloned into RNA interference plasmid. The construction
vector was transfected into ovarian cancer SKOV3 cells by lipofectamine 2000,and the stably transfected cells were isolated by G418 and single clone selection. The downregulating expression of lncRNA
was detected by quantitative real time PCR(qRT-PCR). The characteristics of
transfected SKOV3 cells were analyzed from the assays of invasion and nude mouse tumorigenicity,as well as the expression of snail and E-cadherin mRNA detected by qRT-PCR,and snail detected by immunohistochemistry and Western blot methods in xenograft tumor,respectively.
The lncRNA
expression was proved by RT-PCR in SKOV3 cells. The lncRNA
expression in
transfected SKOV3 cells was significantly lower than the scramble |
| format | Article |
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on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells.
The expression of lncRNA
was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA
gene was cloned into RNA interference plasmid. The construction
vector was transfected into ovarian cancer SKOV3 cells by lipofectamine 2000,and the stably transfected cells were isolated by G418 and single clone selection. The downregulating expression of lncRNA
was detected by quantitative real time PCR(qRT-PCR). The characteristics of
transfected SKOV3 cells were analyzed from the assays of invasion and nude mouse tumorigenicity,as well as the expression of snail and E-cadherin mRNA detected by qRT-PCR,and snail detected by immunohistochemistry and Western blot methods in xenograft tumor,respectively.
The lncRNA
expression was proved by RT-PCR in SKOV3 cells. The lncRNA
expression in
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on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells.
The expression of lncRNA
was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA
gene was cloned into RNA interference plasmid. The construction
vector was transfected into ovarian cancer SKOV3 cells by lipofectamine 2000,and the stably transfected cells were isolated by G418 and single clone selection. The downregulating expression of lncRNA
was detected by quantitative real time PCR(qRT-PCR). The characteristics of
transfected SKOV3 cells were analyzed from the assays of invasion and nude mouse tumorigenicity,as well as the expression of snail and E-cadherin mRNA detected by qRT-PCR,and snail detected by immunohistochemistry and Western blot methods in xenograft tumor,respectively.
The lncRNA
expression was proved by RT-PCR in SKOV3 cells. The lncRNA
expression in
transfected SKOV3 cells was significantly lower than the scramble</description><subject>Animals</subject><subject>Cadherins - metabolism</subject><subject>Carcinoma, Ovarian Epithelial - genetics</subject><subject>Carcinoma, Ovarian Epithelial - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Female</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neoplasm Invasiveness</subject><subject>Ovarian Neoplasms</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Small Interfering</subject><subject>Snail Family Transcription Factors - metabolism</subject><subject>Transfection</subject><issn>1672-173X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEFrwjAcxXPYmOL8CiPHXQqJMfm3RyluCk5BetitJGniAm3aJa3gt1_G3Onx4L0fj_eA5lTAKqPAPmdoGaNThFBKWJHDE5qxZNaCsTmKW2uNHiPuLa5kuJjR-QtuvT4fN3h3qjb7M-49Hr8M3vurjC4Z6Rt8nBqDP_opGlxNXR_cxXin3Xj7BW0Hlwqtky0-XWVw0uNSem0CLk3bxmf0aGUbzfKuC1S9batylx1O7_tyc8gGLlgmVmsqtNKWSiWLhgPhlmuwhVIN05zmghV6bXJoNNECgBKtAHhOC6AScs4W6PUPO4T-ezJxrDsXdRogvUnD6xUBmqg50BR9uUcn1ZmmHoLrZLjV_z-xH4RCY2c</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Zhou, Yu-Fu</creator><creator>Chen, Deng-Yu</creator><creator>Chu, Yi-Fan</creator><creator>Zheng, Qing-Wei</creator><creator>Xu, Zhi-Ben</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201805</creationdate><title>Effects of Targeting lncRNA HOTAIR on the Invasion and Nude Mouse Tumorigenicity of Epithelial Ovarian Cancer Cells</title><author>Zhou, Yu-Fu ; Chen, Deng-Yu ; Chu, Yi-Fan ; Zheng, Qing-Wei ; Xu, Zhi-Ben</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p563-62416cbcf1aba9d5705f5c7f9bbd3c518639c4e87dc0c67710cb77581971a7853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Cadherins - metabolism</topic><topic>Carcinoma, Ovarian Epithelial - genetics</topic><topic>Carcinoma, Ovarian Epithelial - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Female</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Neoplasm Invasiveness</topic><topic>Ovarian Neoplasms</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Small Interfering</topic><topic>Snail Family Transcription Factors - metabolism</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Yu-Fu</creatorcontrib><creatorcontrib>Chen, Deng-Yu</creatorcontrib><creatorcontrib>Chu, Yi-Fan</creatorcontrib><creatorcontrib>Zheng, Qing-Wei</creatorcontrib><creatorcontrib>Xu, Zhi-Ben</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Yu-Fu</au><au>Chen, Deng-Yu</au><au>Chu, Yi-Fan</au><au>Zheng, Qing-Wei</au><au>Xu, Zhi-Ben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Targeting lncRNA HOTAIR on the Invasion and Nude Mouse Tumorigenicity of Epithelial Ovarian Cancer Cells</atitle><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle><addtitle>Sichuan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2018-05</date><risdate>2018</risdate><volume>49</volume><issue>3</issue><spage>352</spage><epage>357</epage><pages>352-357</pages><issn>1672-173X</issn><abstract>To explore the effects of shRNA-mediated downregulating lncRNA
on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells.
The expression of lncRNA
was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA
gene was cloned into RNA interference plasmid. The construction
vector was transfected into ovarian cancer SKOV3 cells by lipofectamine 2000,and the stably transfected cells were isolated by G418 and single clone selection. The downregulating expression of lncRNA
was detected by quantitative real time PCR(qRT-PCR). The characteristics of
transfected SKOV3 cells were analyzed from the assays of invasion and nude mouse tumorigenicity,as well as the expression of snail and E-cadherin mRNA detected by qRT-PCR,and snail detected by immunohistochemistry and Western blot methods in xenograft tumor,respectively.
The lncRNA
expression was proved by RT-PCR in SKOV3 cells. The lncRNA
expression in
transfected SKOV3 cells was significantly lower than the scramble</abstract><cop>China</cop><pmid>30014633</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1672-173X |
| ispartof | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2018-05, Vol.49 (3), p.352-357 |
| issn | 1672-173X |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cadherins - metabolism Carcinoma, Ovarian Epithelial - genetics Carcinoma, Ovarian Epithelial - pathology Cell Line, Tumor Cell Proliferation Female Humans Mice Mice, Nude Neoplasm Invasiveness Ovarian Neoplasms RNA, Long Noncoding - genetics RNA, Small Interfering Snail Family Transcription Factors - metabolism Transfection |
| title | Effects of Targeting lncRNA HOTAIR on the Invasion and Nude Mouse Tumorigenicity of Epithelial Ovarian Cancer Cells |
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