Effects of Targeting lncRNA HOTAIR on the Invasion and Nude Mouse Tumorigenicity of Epithelial Ovarian Cancer Cells

To explore the effects of shRNA-mediated downregulating lncRNA on the invasion,nude mouse tumorigenicity and snail expression of epithelial ovarian cancer SKOV3 cells. The expression of lncRNA was detected by RT-PCR in SKOV3 cells. The shRNA targeting the lncRNA gene was cloned into RNA interference plasmid. The construction vector was transfected into ovarian cancer SKOV3 cells by lipofectamine 2000,and the stably transfected cells were isolated by G418 and single clone selection. The downregulating expression of lncRNA was detected by quantitative real time PCR(qRT-PCR). The characteristics of transfected SKOV3 cells were analyzed from the assays of invasion and nude mouse tumorigenicity,as well as the expression of snail and E-cadherin mRNA detected by qRT-PCR,and snail detected by immunohistochemistry and Western blot methods in xenograft tumor,respectively. The lncRNA expression was proved by RT-PCR in SKOV3 cells. The lncRNA expression in transfected SKOV3 cells was significantly lower than the scramble