The Production of Cytopathic Changes in Canine Cell Lines by Infectious Agents: Cell Lines Derived from Melanoma and Thyroidal Carcinoma

The cytopathic effect of more than 30 viruses and Toxoplasma gondii and Histoplasma capsulatum was determined in established canine thyroid adenocarcinoma and canine melanoma cell lines. Marked effect was observed with the viruses of canine hepatitis, pseudorabies, and canine distemper, and reo type 1 and vaccinia viruses in both cell lines. Moderate effect was found with human adenovirus type 1, swine adenovirus and the viruses of swine influenza and Newcastle disease. Cytopathic changes were produced with the virus of herpes simplex in the thyroid adenocarcinoma cells. A transient giant cell formation was produced by the virus of measles in the thyroid cell line. Hemadsorption was demonstrated in both cell lines with vaccinia virus. Toxoplasma gondii multiplied in both cell lines and produced cellular degeneration and necrosis. Histoplasma capsulatum grew in the thyroid adenocarcinoma cell line with little cellular degeneration.