Actinomadura hankyongense sp. nov. Isolated From Soil of Ginseng Cultivating Field

A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 556 T ) was isolated from soil of a ginseng field and subjected to its taxonomic position. Based on 16S rRNA gene sequence similarity, strain Gsoil 556 T was shown to belong to the genus Actinomadura of the family Thermomonosporaceae and was closely related to A. montaniterrae CYP1-1B T (99.3%), A. nitritigenes DSM 44137 T (98.7%), and A. rudentiformis HMC1 T (98.5%), while it showed less than 98.4% sequence similarity to the other species of this genus. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that it is most closely related to A. rudentiformis HMC1 T and A. nitritigenes DSM 44137 T . The DNA G+C content was 73.1 mol%. The peptidoglycan was meso-diaminopimelic acid and the whole-cell sugar contained fucose, galactose, glucose, mannose, and ribose. The predominant menaquinone (KK) was MK-9(H 8 ) [55%] and MK-9(H 6 ) [45%]. The major cellular fatty acids were C 14:0 , C 16:0 , C 18:1 ω 9 c and summed feature 3 (C 16:1 ω 6 c /C 16:1 ω 7 c ). All these data supported the affiliation of strain Gsoil 556 T to the genus Actinomadura . The DNA–DNA hybridization between strain Gsoil 556 T and its phylogenetically closest relatives were less than 40%. Furthermore, the results of physiological and biochemical tests enabled strain Gsoil 556 T to be differentiated genotypically and phenotypically from currently known Actinomadura species. Therefore, strain Gsoil 556 T represents a novel species of the genus Actinomadura , for which the name Actinomadura hankyongense sp. nov. is proposed. The type strain Gsoil 556 T (=KACC 19438 T =LMG 30327 T ).