Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus
Aim To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials. Methodology Sixty polyethylene tubes filled with Biodentine or MTA, and...
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| Veröffentlicht in: | International endodontic journal 2019-01, Vol.52 (1), p.54-67 |
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| creator | Fonseca, T. S. Silva, G. F. Guerreiro‐Tanomaru, J. M. Sasso‐Cerri, E. Tanomaru‐Filho, M. Cerri, P. S. |
| description | Aim
To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials.
Methodology
Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively. FGF‐1 and Ki‐67 were detected by immunohistochemistry, and the number of immunolabelled cells was computed. The collagen content was estimated in the picrosirius red‐stained sections. The data were subjected to two‐way ANOVA followed by Tukey's test (P ≤ 0.05).
Results
The capsules were associated with a significant increase (P |
| doi_str_mv | 10.1111/iej.12981 |
| format | Article |
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To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials.
Methodology
Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively. FGF‐1 and Ki‐67 were detected by immunohistochemistry, and the number of immunolabelled cells was computed. The collagen content was estimated in the picrosirius red‐stained sections. The data were subjected to two‐way ANOVA followed by Tukey's test (P ≤ 0.05).
Results
The capsules were associated with a significant increase (P < 0.0001) in the number of fibroblasts and mast cells, and in the collagen content over time. A significant decrease (P < 0.0001) in the immunoexpression of FGF‐1 and Ki‐67 was observed in all groups from the 7th–60th day. At 60 days, the number of fibroblasts (P = 0.0226) and the collagen content (P < 0.0001) were significantly greater in MTA than Biodentine specimens, while the greatest number of mast cells and FGF‐1‐immunolabelled cells was observed in Biodentine specimens (P < 0.0001). A significant difference in Ki‐67 immunoexpression was not detected between specimens of Biodentine and MTA.
Conclusions
The collagen‐rich capsule formed slowly around Biodentine in comparison with MTA. FGF‐1 and mast cells participated in capsule remodelling, stimulating fibroblast proliferation and subsequent collagen production, in response to subcutaneous implants.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12981</identifier><identifier>PMID: 29975794</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; biocompatibility ; Bismuth - pharmacology ; Calcium Compounds - pharmacology ; Cell proliferation ; Cell Proliferation - drug effects ; Collagen ; Collagen - metabolism ; Dentistry ; Endodontics ; Fibroblast Growth Factor 1 - metabolism ; fibroblast growth factor‐1 ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - pathology ; Histamine ; Immunohistochemistry ; Implants, Experimental ; Ki-67 Antigen - metabolism ; Male ; Mast cells ; Mast Cells - drug effects ; Mast Cells - immunology ; Mast Cells - metabolism ; Mast Cells - pathology ; Materials Testing ; Oxides - pharmacology ; Paraffin ; Polyethylene ; Rats ; Root Canal Filling Materials - pharmacology ; Silicates - pharmacology ; Subcutaneous Tissue - drug effects ; Subcutaneous Tissue - immunology ; Subcutaneous Tissue - metabolism</subject><ispartof>International endodontic journal, 2019-01, Vol.52 (1), p.54-67</ispartof><rights>2018 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2018 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3881-9a77fc244b7e447b5bd30217dca07c95d13314f181a507eec661a21cd0e383c83</citedby><cites>FETCH-LOGICAL-c3881-9a77fc244b7e447b5bd30217dca07c95d13314f181a507eec661a21cd0e383c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.12981$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.12981$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29975794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fonseca, T. S.</creatorcontrib><creatorcontrib>Silva, G. F.</creatorcontrib><creatorcontrib>Guerreiro‐Tanomaru, J. M.</creatorcontrib><creatorcontrib>Sasso‐Cerri, E.</creatorcontrib><creatorcontrib>Tanomaru‐Filho, M.</creatorcontrib><creatorcontrib>Cerri, P. S.</creatorcontrib><title>Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials.
Methodology
Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively. FGF‐1 and Ki‐67 were detected by immunohistochemistry, and the number of immunolabelled cells was computed. The collagen content was estimated in the picrosirius red‐stained sections. The data were subjected to two‐way ANOVA followed by Tukey's test (P ≤ 0.05).
Results
The capsules were associated with a significant increase (P < 0.0001) in the number of fibroblasts and mast cells, and in the collagen content over time. A significant decrease (P < 0.0001) in the immunoexpression of FGF‐1 and Ki‐67 was observed in all groups from the 7th–60th day. At 60 days, the number of fibroblasts (P = 0.0226) and the collagen content (P < 0.0001) were significantly greater in MTA than Biodentine specimens, while the greatest number of mast cells and FGF‐1‐immunolabelled cells was observed in Biodentine specimens (P < 0.0001). A significant difference in Ki‐67 immunoexpression was not detected between specimens of Biodentine and MTA.
Conclusions
The collagen‐rich capsule formed slowly around Biodentine in comparison with MTA. FGF‐1 and mast cells participated in capsule remodelling, stimulating fibroblast proliferation and subsequent collagen production, in response to subcutaneous implants.</description><subject>Animals</subject><subject>biocompatibility</subject><subject>Bismuth - pharmacology</subject><subject>Calcium Compounds - pharmacology</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Fibroblast Growth Factor 1 - metabolism</subject><subject>fibroblast growth factor‐1</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - pathology</subject><subject>Histamine</subject><subject>Immunohistochemistry</subject><subject>Implants, Experimental</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Male</subject><subject>Mast cells</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - immunology</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Materials Testing</subject><subject>Oxides - pharmacology</subject><subject>Paraffin</subject><subject>Polyethylene</subject><subject>Rats</subject><subject>Root Canal Filling Materials - pharmacology</subject><subject>Silicates - pharmacology</subject><subject>Subcutaneous Tissue - drug effects</subject><subject>Subcutaneous Tissue - immunology</subject><subject>Subcutaneous Tissue - metabolism</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EokvhwAsgS1zgkNZjx3FyXKpuKbTiUs6W40yKV4m9xLFKb5w58Yw8Ce5m4YCEL2NpPn2amZ-Ql8BOIL9Th9sT4E0Nj8gKRCULLht4TFYMSlHwupZH5FmMW8aYZAKekiPeNEqqplyRH9cmztTiMERqfEfdOCYf8Ntuwhhd8DT0dHOx-fX9J-z7H13-Voo6Tycz05ham2bjMaRIZxdjQtqHYQh3zt_S-Qtm4W4wfjbzQfbOhQ797Dzufdc3a7r2tzik-Jw86c0Q8cWhHpPPm_Obs_fF1aeLy7P1VWFFXUPRGKV6y8uyVViWqpVtJxgH1VnDlG1kB0JA2UMNRjKFaKsKDAfbMRS1sLU4Jm8W724KXxPGWY8uPlxgWUNzVlWlEo2UGX39D7oNafJ5Os1BMq5EzcpMvV0oO4UYJ-z1bnKjme41MP0QkM4B6X1AmX11MKZ2xO4v-SeRDJwuwJ0b8P7_Jn15_mFR_ga-_5t6</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Fonseca, T. S.</creator><creator>Silva, G. F.</creator><creator>Guerreiro‐Tanomaru, J. M.</creator><creator>Sasso‐Cerri, E.</creator><creator>Tanomaru‐Filho, M.</creator><creator>Cerri, P. S.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201901</creationdate><title>Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus</title><author>Fonseca, T. S. ; Silva, G. F. ; Guerreiro‐Tanomaru, J. M. ; Sasso‐Cerri, E. ; Tanomaru‐Filho, M. ; Cerri, P. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3881-9a77fc244b7e447b5bd30217dca07c95d13314f181a507eec661a21cd0e383c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>biocompatibility</topic><topic>Bismuth - pharmacology</topic><topic>Calcium Compounds - pharmacology</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Fibroblast Growth Factor 1 - metabolism</topic><topic>fibroblast growth factor‐1</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - pathology</topic><topic>Histamine</topic><topic>Immunohistochemistry</topic><topic>Implants, Experimental</topic><topic>Ki-67 Antigen - metabolism</topic><topic>Male</topic><topic>Mast cells</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - immunology</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - pathology</topic><topic>Materials Testing</topic><topic>Oxides - pharmacology</topic><topic>Paraffin</topic><topic>Polyethylene</topic><topic>Rats</topic><topic>Root Canal Filling Materials - pharmacology</topic><topic>Silicates - pharmacology</topic><topic>Subcutaneous Tissue - drug effects</topic><topic>Subcutaneous Tissue - immunology</topic><topic>Subcutaneous Tissue - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fonseca, T. S.</creatorcontrib><creatorcontrib>Silva, G. F.</creatorcontrib><creatorcontrib>Guerreiro‐Tanomaru, J. M.</creatorcontrib><creatorcontrib>Sasso‐Cerri, E.</creatorcontrib><creatorcontrib>Tanomaru‐Filho, M.</creatorcontrib><creatorcontrib>Cerri, P. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fonseca, T. S.</au><au>Silva, G. F.</au><au>Guerreiro‐Tanomaru, J. M.</au><au>Sasso‐Cerri, E.</au><au>Tanomaru‐Filho, M.</au><au>Cerri, P. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2019-01</date><risdate>2019</risdate><volume>52</volume><issue>1</issue><spage>54</spage><epage>67</epage><pages>54-67</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials.
Methodology
Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively. FGF‐1 and Ki‐67 were detected by immunohistochemistry, and the number of immunolabelled cells was computed. The collagen content was estimated in the picrosirius red‐stained sections. The data were subjected to two‐way ANOVA followed by Tukey's test (P ≤ 0.05).
Results
The capsules were associated with a significant increase (P < 0.0001) in the number of fibroblasts and mast cells, and in the collagen content over time. A significant decrease (P < 0.0001) in the immunoexpression of FGF‐1 and Ki‐67 was observed in all groups from the 7th–60th day. At 60 days, the number of fibroblasts (P = 0.0226) and the collagen content (P < 0.0001) were significantly greater in MTA than Biodentine specimens, while the greatest number of mast cells and FGF‐1‐immunolabelled cells was observed in Biodentine specimens (P < 0.0001). A significant difference in Ki‐67 immunoexpression was not detected between specimens of Biodentine and MTA.
Conclusions
The collagen‐rich capsule formed slowly around Biodentine in comparison with MTA. FGF‐1 and mast cells participated in capsule remodelling, stimulating fibroblast proliferation and subsequent collagen production, in response to subcutaneous implants.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29975794</pmid><doi>10.1111/iej.12981</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals biocompatibility Bismuth - pharmacology Calcium Compounds - pharmacology Cell proliferation Cell Proliferation - drug effects Collagen Collagen - metabolism Dentistry Endodontics Fibroblast Growth Factor 1 - metabolism fibroblast growth factor‐1 Fibroblasts Fibroblasts - drug effects Fibroblasts - pathology Histamine Immunohistochemistry Implants, Experimental Ki-67 Antigen - metabolism Male Mast cells Mast Cells - drug effects Mast Cells - immunology Mast Cells - metabolism Mast Cells - pathology Materials Testing Oxides - pharmacology Paraffin Polyethylene Rats Root Canal Filling Materials - pharmacology Silicates - pharmacology Subcutaneous Tissue - drug effects Subcutaneous Tissue - immunology Subcutaneous Tissue - metabolism |
| title | Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus |
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