Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

Aim To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor‐1 (FGF‐1) and mast cells in the tissue response to these endodontic materials. Methodology Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively. FGF‐1 and Ki‐67 were detected by immunohistochemistry, and the number of immunolabelled cells was computed. The collagen content was estimated in the picrosirius red‐stained sections. The data were subjected to two‐way ANOVA followed by Tukey's test (P ≤ 0.05). Results The capsules were associated with a significant increase (P