GS18
PROTEOMIC CHANGES IN MESENTERIC LYMPH SECONDARY TO HAEMORRHAGIC SHOCK

Background: Biologically active factors produced by the intestine and transported by the protein fraction of mesenteric lymph are thought to contribute significantly to the development of distant organ failure in haemorrhagic shock. Despite the likely relevance of the protein composition of mesenteric lymph conditioned by haemorrhagic shock, there is no detailed description of its proteome. The aim of this study was to provide the first comprehensive description of the proteome of haemorrhagic shock conditioned mesenteric lymph. Methods: 20 male Wistar rats were randomized to either control or haemorrhagic shock group. Mean arterial pressure was reduced to 30 mmHg in the shock group for 90 minutes followed by resuscitation and mesenteric lymph collection for another 90 minutes. Proteomic changes in mesenteric lymph due to haemorrhagic shock were identified using LC‐MS/MS. Results: 245 proteins were identified in mesenteric lymph, including 35 hypothetical proteins. 60 of the 245 proteins had a significant increase in their relative abundance in the haemorrhagic shock group vs. sham group. Potentially toxic pancreatic enzymes had increased abundance in post‐shock mesenteric lymph. In addition, a bioinformatics approach allowed for the identification of key pathways relating to defence and metabolic responses. A number of potentially important proteins were also identified as targets for future research in hemorrhagic shock: peroxiredoxins, 14‐3‐3 zeta/epsilon, calmodulin, cofilin, and profilin. Conclusions: This study not only provides the first comprehensive list of protein changes in the haemorrhagic shock conditioned mesenteric lymph, but also provides rational for future research.