Calcium carbonate microcapsules encapsulating biomacromolecules

This paper reports the preparation of CaCO 3 microcapsules and the direct encapsulation of biomacromolecules such as bovine serum albumin (BSA) and duplex DNA into the CaCO 3 microcapsules. Vacant CaCO 3 microcapsules were effectively obtained by interfacial reaction method using carbonate salts and calcium salts, which were dissolved in inner water phase or outer one, respectively. For the fabrication of microcapsule structure, the formation of vaterite as a metastable phase of calcium carbonate crystal was an important factor. When some biomacromolecules were dissolved in the aqueous solution of (NH 4) 2CO 3 as the inner water phase, these macromolecules were successfully encapsulated into CaCO 3 microcapsules. Biomacromolecules included in the microcapsules scarcely eliminated without the fracture of the microcapsule particles. These properties of encapsulated biomacromolecules might be utilized in various bio-related materials.