Fluorescence time-resolved macroimaging

While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging of large biological samples that is often required for biomedical applications. Here we present a system that all...

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Veröffentlicht in:Optics letters 2018-07, Vol.43 (13), p.3152-3155
Hauptverfasser: Shcheslavskiy, Vladislav I, Shirmanova, Marina V, Dudenkova, Varvara V, Lukyanov, Konstantin A, Gavrina, Alena I, Shumilova, Anastasia V, Zagaynova, Elena, Becker, Wolfgang
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Sprache:eng
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Zusammenfassung:While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging of large biological samples that is often required for biomedical applications. Here we present a system that allows performing FLIM on macroscopic samples as large as 18 mm with a lateral resolution of 15 μm. The performance of the system is verified with FLIM of endogenous metabolic cofactor reduced nicotinamide adenine dinucleotide (phosphate), NAD(P)H, and genetically encoded fluorescent protein mKate2 in a mouse tumor in vivo.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.43.003152