Increased Receptor Binding by Bovine (b) TSH Bound to Monoclonal Antibody to bTSHβ-subunit

Increased Receptor Binding by Bovine (b) TSH Bound to Monoclonal Antibody to bTSHβ-subunit

TSH receptor (R) binding and cAMP production by bovine (b) TSH-bound to a monoclonal antibody (MoAb) or polyclonal antibody (Ab) to bTSH were examined, using TSH receptor (R) coating tube and porcine thyroid cells. 125 I-bTSH bound-to MoAbs to bTSH(α) or discontinuous type MoAb showed TSHR binding (...

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Veröffentlicht in:Endocrine Journal 2008, Vol.55(1), pp.105-111
Hauptverfasser: OCHI, Yukio, HAMAZU, Masanari, KAJITA, Yoshihiro, HACHIYA, Takashi, KUNIMATSU, Mitoshi, SATO, Takaaki, NAGATA, Atsuo
Format: Artikel
Sprache:eng
Schlagworte:
cAMP
Glycoprotein hormone
Monoclonal antibody to TSH
TSH
TSH receptor assay
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Zusammenfassung:TSH receptor (R) binding and cAMP production by bovine (b) TSH-bound to a monoclonal antibody (MoAb) or polyclonal antibody (Ab) to bTSH were examined, using TSH receptor (R) coating tube and porcine thyroid cells. 125 I-bTSH bound-to MoAbs to bTSH(α) or discontinuous type MoAb showed TSHR binding (10%) similar to intact 125 I-bTSH. TSHR binding was completely decreased (
ISSN:0918-8959
1348-4540
DOI:10.1507/endocrj.K07E-015