Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells
Objectives The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival....
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Veröffentlicht in: | Biotechnology letters 2018-08, Vol.40 (8), p.1237-1244 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Objectives
The objective of this study was to evaluate the ability of
Lactobacillus curvatus
CRL705, CRL1532, and CRL1533 and
Lactobacillus sakei
CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that
Lactobacill
us spp. can modulate the ability of
Listeria monocytogenes
FBUNT to adhere to and invade Caco-2 cells.
Results
Lactobacillus
strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions. All strains hydrolyzed bile salts using chenodeoxycholic acid as a substrate and adhered to Caco-2 cells. Cell-free supernatants (CFSs) showed antimicrobial activity against
L. monocytogenes
as demonstrated by agar diffusion assays. The average percentages of
L. monocytogenes
adhesion decreased from 67.74 to 41.75 and 38.7% in the presence of 50 and 90% (v/v), respectively, for all CFSs tested. The highest concentrations of CFSs completely inhibited the
L. monocytogenes
invasion of Caco-2 cells.
Conclusions
The studied
Lactobacillus
strains have protective effects against the adhesion and invasion of
L. monocytogenes
FBUNT. Alginate encapsulation of these bacteria improved gastrointestinal tolerance such that they could be further studied as potential probiotics against intestinal pathogenic bacteria. |
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ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1007/s10529-018-2572-x |