Selinidin Suppresses IgE-Mediated Mast Cell Activation by Inhibiting Multiple Steps of Fc[epsilon]RI Signaling

IgE-mediated mast cell activation is critical for development of allergic inflammation. We have recently found that selinidin, one of the coumarin derivatives isolated from Angelica keiskei, attenuates mast cell degranulation following engagement of the high-affinity receptor for IgE (Fc[straight epsilon]RI) with IgE and antigen. In the present study, we investigated the effects of selinidin on intracellular signaling and mast cell activation employing bone marrow-derived mast cells. Here, we report that selinidin attenuates the release of β-hexosaminidase, synthesis of leukotriene C4, and production of tumor necrosis factor-α without affecting IgE-Fc[straight epsilon]RI binding. Furthermore, biochemical analyses of the Fc[straight epsilon]RI-mediated signaling pathway demonstrated that selinidin decreases phosphorylation of phospholipase C-γ1, p38 mitogen-activated protein kinase, and IκB-α upon Fc[straight epsilon]RI stimulation. These results suggest that this compound suppresses IgE-mediated mast cell activation by inhibiting multiple steps of Fc[straight epsilon]RI-dependent signaling pathways and would be beneficial for the prevention of allergic inflammation.