The reduced brood nursing by mite-infested honey bees depends on their accelerated behavioral maturation

[Display omitted] •Nurse bees infested by the Varroa mite at the pupal stage are less involved in tending larvae.•Varroa affects JHIII titre, glands development, learning ability and attraction to brood.•Premature transition to foraging by mite-infested bees is confirmed on a physiological basis. The parasitic mite Varroa destructor is regarded as the most important parasite of honey bees and plays a fundamental role in the decline of bee colonies observed in the last decade in the Northern hemisphere. Parasitization has a number of detrimental effects on bees, including reduced nursing, which can have important impacts on colony balance. In this work we investigated at the individual level the causes of this abnormal behavior and found that the reduced nursing activity in mite-infested workers is associated with impaired learning performance and a series of physiological traits that are typical of foragers, including reduced response to brood pheromone, limited development of hypopharyngeal glands and higher juvenile hormone titre in the haemolymph. Altogether our data confirm the premature transition to foraging already postulated based on previous genomics studies, from a physiological point of view.