Structures of O-specific polysaccharides of Pseudomonas psyhrotolerans BIM B-1158G

Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2 [Display omitted] •Lipopolysaccharide was isolated from Pseudomonas psyhrotolerans B-1158G.•Two O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide.•Structures of the O-polysaccharides were established by chemical methods and NMR spectroscopy.