Development of Pyrosequencing Assay for Evaluation of Genetic Stability of Vaccine Strains of Live Attenuated Influenza Type B Vaccine

Development of Pyrosequencing Assay for Evaluation of Genetic Stability of Vaccine Strains of Live Attenuated Influenza Type B Vaccine

We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bulletin of experimental biology and medicine 2018-06, Vol.165 (2), p.243-247
Hauptverfasser: Stepanova, E. А., Krutikova, E. V., Kiseleva, I. V., Vasil’eva, V. A., Rudenko, L. G.
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Biomedical and Life Sciences
Biomedicine
Cell Biology
Clinical trials
DNA sequencing
Genetic analysis
Genomes
Genomics
Health aspects
Influenza
Influenza B
Influenza vaccines
Influenza viruses
Internal Medicine
Laboratory Medicine
Mutation
Pathology
Vaccination
Vaccines
Virology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be used for analysis of genetic stability of reassortants during vaccine strain development and manufacturing, as well as genetic stability of vaccine isolates of influenza B virus during pre-clinical and clinical trials.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4139-1